S100A8/A9 Complex (Calprotectin) Antikörper

Produktnummer ABIN2192016

Dieses Anti-S100A8/A9 Complex (Calprotectin)-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von S100A8/A9 Complex (Calprotectin) in FACS, WB und HPLC. Geeignet für Human. Dieses Primary Antibody wurde in 4+ Publikationen zitiert.

Kurzübersicht für S100A8/A9 Complex (Calprotectin) Antikörper (ABIN2192016)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser S100A8/A9 Complex (Calprotectin) Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), High Pressure Liquid Chromatography (HPLC)

Klon: 27E10

Produktdetails anti-S100A8/A9 Complex (Calprotectin) Antikörper

Kreuzreaktivität (Details): Cross reactivity: Mouse : No, Rhesus Monkey : Yes (subpopulation of macrophages)

Sterilität: 0.2 μm filtered

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive Human granulocytes control Negative Platelets, lymphocytes, HL-60 cells control

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Haltbarkeit: 12 months

Referenzen für anti-S100A8/A9 Complex (Calprotectin) Antikörper (ABIN2192016)

Champaiboon, Sappington, Guenther, Ross, Herzberg: "Calprotectin S100A9 calcium-binding loops I and II are essential for keratinocyte resistance to bacterial invasion." in: The Journal of biological chemistry, Vol. 284, Issue 11, pp. 7078-90, (2009) (PubMed).

Kuhn, Sonntag, Sunderkötter, Lehmann, Vestweber, Ruzicka: "Upregulation of epidermal surface molecule expression in primary and ultraviolet-induced lesions of lupus erythematosus tumidus." in: The British journal of dermatology, Vol. 146, Issue 5, pp. 801-9, (2002) (PubMed).

Hessian, Fisher: "The heterodimeric complex of MRP-8 (S100A8) and MRP-14 (S100A9). Antibody recognition, epitope definition and the implications for structure." in: European journal of biochemistry / FEBS, Vol. 268, Issue 2, pp. 353-63, (2001) (PubMed).

Zwadlo, Schlegel, Sorg: "A monoclonal antibody to a subset of human monocytes found only in the peripheral blood and inflammatory tissues." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 2, pp. 512-8, (1986) (PubMed).

Details zu S100A8/A9 Complex (Calprotectin)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Andere Bezeichnung: Calprotectin

Hintergrund: The monoclonal antibody 27E10 recognizes an epitope specific for the S100A8/A9 heterocomplex that is not exposed on the individual subunits S100A8 (MRP8, calgranulin-A) or S100A9 (MRP14, calgranulin- B). The calcium-binding migration inhibitory factor-related proteins, MRP-8 (S100A8) and MRP-14 (S100A9) belong to the S100 protein family. The expression of these proteins is largely confined to the cytosol of neutrophils and monocytes. The complex formation of these proteins is a calcium-dependent process. The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60 % of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions. The monoclonal antibody 27E10 can be used for early detection of inflammatory macrophages, for the characterization of tumorous tissues and the monitoring of peripheral blood cell cultures. The antibody 27E10 does not react with lymphocytes or platelets. Aliases S100A8/A9, MRP-8/MRP-14, calprotectin, calgranulin-A/calgranulin-B, L1-protein Immunogen Human blood monocytes

Pathways: S100 Proteine