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PROCR Antikörper (Protein C Receptor, Endothelial) Primary Antibody

PROCR Reaktivität: Human Func, FACS, IP, WB Wirt: Ratte Monoclonal RCR-379 unconjugated
Pubmed (1 reference)
Produktnummer ABIN2192006
$867.55
Zzgl. Versandkosten $45.00
100 μg
Lieferung in 6 bis 8 Werktagen
  • Target Alle PROCR Antikörper anzeigen
    PROCR
    Reaktivität
    • 59
    • 13
    • 12
    • 1
    Human
    Wirt
    • 49
    • 12
    • 11
    Ratte
    Klonalität
    • 51
    • 21
    Monoklonal
    Konjugat
    • 45
    • 12
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser PROCR Antikörper ist unkonjugiert
    Applikation
    • 51
    • 32
    • 21
    • 17
    • 12
    • 10
    • 9
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
    Sterilität
    0.2 μm filtered
    Klon
    RCR-379
  • Applikationshinweise
    For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For inhibition of biological activity dilutions have to be made according to the amounts EPCR to be inhibited.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Buffer
    PBS, containing 0.1 % bovine serum albumin.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
    Haltbarkeit
    12 months
  • Ye, Fukudome, Tsuneyoshi, Satoh, Tokunaga, Sugawara, Mizokami, Kimoto: "The endothelial cell protein C receptor (EPCR) functions as a primary receptor for protein C activation on endothelial cells in arteries, veins, and capillaries." in: Biochemical and biophysical research communications, Vol. 259, Issue 3, pp. 671-7, (1999) (PubMed).

  • Target
    PROCR
    Andere Bezeichnung
    Endothelial Cell Protein C Receptor (PROCR Produkte)
    Synonyme
    PROCR, CCCA, CCD41, EPCR, AI325044, Ccca, Ccd41, Epcr, protein C receptor, protein C receptor, endothelial, PROCR, Procr
    Hintergrund
    The monoclonal antibody RCR-252 recognizes human endothelial protein C receptor (EPCR), a highly glycosylated type I transmembrane protein of 221-amino-acids. These amino acids comprise an extracellular domain, a 25-aa transmembrane domain, and a short (3 aa) intracytoplasmic sequence coding for an ~46 kDa protein. Deglycosylation will reduce the protein mass to 25 kDa. EPCR is expressed strongly on the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney. EPCR is the receptor for protein C, a key player in the anticoagulation pathway. The protein C anticoagulant pathway serves as a major system for controling thrombosis, limiting inflammatory responses, and potentially decreasing endothelial cell apoptosis in response to inflammatory cytokines and ischemia. The essential components of the pathway include thrombin, thrombomodulin, the endothelial cell protein C receptor (EPCR), protein C and protein S. The pathway is initiated when thrombin binds to thrombomodulin on the surface of endothelium. EPCR augments protein C activation by binding protein C and presenting it to the thrombin-thrombomodulin activation complex. Activated protein C (aPC) retains its ability to bind EPCR, and this complex appears to be involved in some of the cellular signaling mechanisms that down-regulate inflammatory cytokine formation (TNF, IL-6). EPCR is shed from the vasculature by inflammatory mediators and thrombin. EPCR binds to activated neutrophils in a process that involves proteinase 3 and Mac-1. Furthermore, EPCR can undergo translocation from the plasma membrane to the nucleus. EPCR can be cleaved to release a soluble form (sEPCR) in the circulation. This sEPCR is detected as a single species of 43 kDa, resulting from shedding of membrane EPCR by the action of a metalloprotease, which is stimulated by thrombin and by some inflammatory mediators. Soluble EPCR binds PC and aPC with similar affinity, but its binding to aPC inhibits the anticoagulant activity of aPC by blocking its binding to phospholipids and by abrogating its ability to inactivate factor Va. sEPCR can be detected in plasma. In normal persons, sEPCR is present in levels of 83.6 +/- 17.2 ng/mL. Elevated levels of sEPCR are positively correlated to a higher risk for thrombosis. Furthermore, a haplotype (A3 allele) has been linked to elevated levels of sEPCR (264 +/-174 ng/mL).
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