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FHL2 Antikörper (Four and A Half LIM Domains 2) Primary Antibody

FHL2 Reaktivität: Human IHC (fro), IF, IHC (p), WB Wirt: Maus Monoclonal F4B2-B11 unconjugated
Pubmed (1 reference)
Produktnummer ABIN2192004
$867.55
Zzgl. Versandkosten $45.00
100 μg
Lieferung in 6 bis 8 Werktagen
  • Target Alle FHL2 Antikörper anzeigen
    FHL2
    Reaktivität
    • 51
    • 32
    • 19
    • 6
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Wirt
    • 36
    • 8
    • 8
    Maus
    Klonalität
    • 37
    • 14
    Monoklonal
    Konjugat
    • 28
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Dieser FHL2 Antikörper ist unkonjugiert
    Applikation
    • 37
    • 20
    • 12
    • 7
    • 5
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Sterilität
    0.2 μm filtered
    Klon
    F4B2-B11
  • Applikationshinweise
    For immunohistology and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Buffer
    PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
    Haltbarkeit
    12 months
  • Martin, Schneider, Janetzky, Waibler, Pandur, Kühl, Behrens, von der Mark, Starzinski-Powitz, Wixler: "The LIM-only protein FHL2 interacts with beta-catenin and promotes differentiation of mouse myoblasts." in: The Journal of cell biology, Vol. 159, Issue 1, pp. 113-22, (2002) (PubMed).

  • Target
    FHL2
    Andere Bezeichnung
    Fhl2 (FHL2 Produkte)
    Synonyme
    AAG11, DRAL, FHL-2, SLIM-3, SLIM3, C76204, FHL2, zgc:92428, four and a half LIM domains 2, four and a half LIM domains 2a, FHL2, Fhl2, fhl2, fhl2a
    Hintergrund
    The monoclonal antibody F4B2-B11 reacts specifically with the LIM-only protein FHL2. Five proteins share the same structural organization and a high degree of sequence homology in this group: four and a half LIM domain protein (FHL) 1, FHL2, FHL3, FHL4, and activator of cAMP-responsive element (CRE) modulator (CREM) in testis (ACT). LIM domains are constituted by a conserved cysteine- and histidine- rich structure shaped in two repeated zinc fingers first identified in the proteins encoded by the Lin-11, Isl- 1, and Mec-3 genes. The LIM domain has been shown to function as a protein-protein interaction domain, and has often been described in association with other functional protein motifs, such as homeobox and kinase domains. FHL2 seems to be a promiscuous coactivator because it modulates the activity of the androgen receptor, CRE-binding protein (CREB), and WT1, although some degree of specificity is present because it is unable to stimulate CREM- and Sp1-dependent transcription. FHL2 expression was originally described to be restricted to the heart but it is inducible in other cell types. FHL2 shows specific interaction with beta-catenin, which requires the intact structure of all four LIM domains of FHL2 and the N-terminus plus the first armadillo repeat region of beta-catenin. FHL2 is a muscle-specific repressor of LEF/TCF target genes and promotes myogenic differentiation by interacting with beta-catenin. Monoclonal antibody F4B2-B11 recognizes the N-terminal Zn-Finger motif, it does not crossreact in Western Blotting with the FHL1 and FHL3 proteins. The F4B2-B11 antibody is cross reactive with mouse and rat FHL2.
    Pathways
    Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha
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