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FHL2 Antikörper

Dieses Anti-FHL2-Antikörper ist ein Mouse Monoclonal-Antikörper zur Detektion von FHL2 in WB, IF, IHC (p), IHC (fro). Geeignet für Human. Dieses Primary Antibody wurde in 1 Publikation zitiert.
Produktnummer ABIN2192004

Kurzübersicht für FHL2 Antikörper (ABIN2192004)

Target

Alle FHL2 Antikörper anzeigen
FHL2 (Four and A Half LIM Domains 2 (FHL2))

Reaktivität

  • 45
  • 32
  • 18
  • 5
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Human

Wirt

  • 37
  • 7
  • 2
Maus

Klonalität

  • 32
  • 13
Monoklonal

Konjugat

  • 28
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
Dieser FHL2 Antikörper ist unkonjugiert

Applikation

  • 26
  • 14
  • 13
  • 7
  • 7
  • 6
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon

F4B2-B11
  • Sterilität

    0.2 μm filtered
  • Applikationshinweise

    For immunohistology and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Buffer

    PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C

    Informationen zur Lagerung

    Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

    Haltbarkeit

    12 months
  • Martin, Schneider, Janetzky, Waibler, Pandur, Kühl, Behrens, von der Mark, Starzinski-Powitz, Wixler: "The LIM-only protein FHL2 interacts with beta-catenin and promotes differentiation of mouse myoblasts." in: The Journal of cell biology, Vol. 159, Issue 1, pp. 113-22, (2002) (PubMed).

  • Target

    FHL2 (Four and A Half LIM Domains 2 (FHL2))

    Andere Bezeichnung

    Fhl2

    Hintergrund

    The monoclonal antibody F4B2-B11 reacts specifically with the LIM-only protein FHL2. Five proteins share the same structural organization and a high degree of sequence homology in this group: four and a half LIM domain protein (FHL) 1, FHL2, FHL3, FHL4, and activator of cAMP-responsive element (CRE) modulator (CREM) in testis (ACT). LIM domains are constituted by a conserved cysteine- and histidine- rich structure shaped in two repeated zinc fingers first identified in the proteins encoded by the Lin-11, Isl- 1, and Mec-3 genes. The LIM domain has been shown to function as a protein-protein interaction domain, and has often been described in association with other functional protein motifs, such as homeobox and kinase domains. FHL2 seems to be a promiscuous coactivator because it modulates the activity of the androgen receptor, CRE-binding protein (CREB), and WT1, although some degree of specificity is present because it is unable to stimulate CREM- and Sp1-dependent transcription. FHL2 expression was originally described to be restricted to the heart but it is inducible in other cell types. FHL2 shows specific interaction with beta-catenin, which requires the intact structure of all four LIM domains of FHL2 and the N-terminus plus the first armadillo repeat region of beta-catenin. FHL2 is a muscle-specific repressor of LEF/TCF target genes and promotes myogenic differentiation by interacting with beta-catenin. Monoclonal antibody F4B2-B11 recognizes the N-terminal Zn-Finger motif, it does not crossreact in Western Blotting with the FHL1 and FHL3 proteins. The F4B2-B11 antibody is cross reactive with mouse and rat FHL2.

    Pathways

    Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha
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