Lipid A Antikörper

Produktnummer ABIN2191914

Produktdetails

Target: Lipid A

Reaktivität: Chemical

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB)

Endotoxin-Niveau: Low endotoxin level

Klon: 43

Anwendungsinformationen

Applikationshinweise: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 1 mL(> 200 μg/mL) culture medium with a low endotoxin level containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20 °C Under recommended storage conditions, product is stable for one year.

Haltbarkeit: 12 months

Referenzen

Chou, Chuang, Eng, Chen, Wang, Chen, Lin: "Endotoxin and CD14 in the progression of biliary atresia." in: Journal of translational medicine, Vol. 8, pp. 138, (2011) (PubMed).

Kuhn, Brade, Appelmelk, Kusumoto, Rietschel, Brade: "The antibody reactivity of monoclonal lipid A antibodies is influenced by the acylation pattern of lipid A and the assay system employed." in: Immunobiology, Vol. 189, Issue 5, pp. 457-71, (1994) (PubMed).

Kuhn, Brade, Appelmelk, Kusumoto, Rietschel, Brade: "Characterization of the epitope specificity of murine monoclonal antibodies directed against lipid A." in: Infection and immunity, Vol. 60, Issue 6, pp. 2201-10, (1992) (PubMed).

Antigendetails

Target: Lipid A

Substanzklasse: Chemical

Hintergrund: The monoclonal antibody 43 reacts with the hydrophilic backbone of free lipid A. This antibody only reacts with lipid A not covalent bound to LPS. LPS is chemically composed of a lipid part, lipid A, which is responsible for the endotoxic activities of LPS, and a heteropolysaccharide which generally consists of an oligosaccharide, termed the core region, and, in some bacterial families, of the so-called O chain, composed of repeating oligosaccharide units.