MHC, Class I Antikörper

Produktnummer ABIN2191839

Dieses Anti-MHC, Class I-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von MHC, Class I in FACS und IHC (fro). Geeignet für Maus.

Kurzübersicht für MHC, Class I Antikörper (ABIN2191839)

Target: MHC, Class I

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser MHC, Class I Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: ER-HR52

Produktdetails anti-MHC, Class I Antikörper

Sterilität: 0.2 μm filtered

Anwendungsinformationen

Applikationshinweise: For immunohistology and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Haltbarkeit: 12 months

Details zu MHC, Class I

Target: MHC, Class I

Andere Bezeichnung: Mhc Class I

Hintergrund: The monoclonal antibody ER-HR52 specifically reacts with major histocompatibility complex (MHC) class I antigens of the mouse and, therefore, it is a valuable tool for studying cytotoxic T-cell interactions with class I positive antigen presenting cells. The primary immunological function of MHC molecules is to bind and present antigenic peptides on the surfaces of cells for recognition by the antigen-specific T cell receptors (TCRs) of lymphocytes. MHC class I molecules are expressed on the surfaces of most cells and are recognized by CD8-positive cytotoxic T-cells, an essential step for initiating the elimination of virally infected cells by T-cell-mediated lysis. MHC class I molecules are heterodimers composed of an alpha (44kD) and a beta (beta microglobulin, 11 kD) subunit. The first two structural domains of the alpha subunit associate to form the peptide-binding pocket. The monoclonal antibody ER-HR52 reacts with MHC class I, an antigen that is expressed by all somatic cells at varying levels. Lymphocytes are highly positive, whereas fibroblasts and neurons show only a low level of antigen expression. ER-HR52 recognizes murine MHC class I molecules on the surface of cells of the following haplotypes: H-2Db, H-2Dw16, H-2d,p,q. Weaker reactivity is found in mice of the following haplotypes: H-2f,r,s,w17, w23,w27. MHC class I molecules of other haplotypes are not recognized by ER-MP52. There is no cross-reactivity with human MHC class I molecules.