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nectin-3 Antikörper (Nectin cell adhesion molecule 3) Primary Antibody

NECTIN3 Reaktivität: Maus IA, FACS, IF Wirt: Ratte Monoclonal 103-A1 unconjugated
Pubmed (3 Referenzen)
Produktnummer ABIN2191802
$729.45
Zzgl. Versandkosten $45.00
100 μg
Lieferung in 6 bis 8 Werktagen
  • Target Alle nectin-3 (NECTIN3) Antikörper anzeigen
    nectin-3 (NECTIN3)
    Reaktivität
    • 61
    • 38
    • 8
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Maus
    Wirt
    • 54
    • 8
    • 6
    • 1
    Ratte
    Klonalität
    • 58
    • 11
    Monoklonal
    Konjugat
    • 37
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser nectin-3 Antikörper ist unkonjugiert
    Applikation
    • 49
    • 35
    • 14
    • 13
    • 13
    • 10
    • 9
    • 9
    • 5
    • 5
    • 4
    • 3
    • 3
    • 1
    • 1
    Immunoassay (IA), Flow Cytometry (FACS), Immunofluorescence (IF)
    Kreuzreaktivität (Details)
    Cross reactivity: Human nectin-3 : No, Rat nectin-3 : No
    Sterilität
    0.2 μm filtered
    Klon
    103-A1
    Isotyp
    IgG2a
  • Applikationshinweise
    For immunohistochemistry and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive L-cells (A cultured line of C3H mouse fibroblasts), control Negative Cells/tissues derived from nectin-3 knockout mouse (Ref 5) control
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Buffer
    PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
  • Toyama, Suzuki-Toyota, Maekawa, Ito, Toshimori: "Disruption of ectoplasmic specializations between Sertoli cells and maturing spermatids by anti-nectin-2 and anti-nectin-3 antibodies." in: Asian journal of andrology, Vol. 10, Issue 4, pp. 577-84, (2008) (PubMed).

    Inagaki, Irie, Ishizaki, Tanaka-Okamoto, Morimoto, Inoue, Ohtsuka, Miyoshi, Takai: "Roles of cell-adhesion molecules nectin 1 and nectin 3 in ciliary body development." in: Development (Cambridge, England), Vol. 132, Issue 7, pp. 1525-37, (2005) (PubMed).

    Guttman, Takai, Vogl: "Evidence that tubulobulbar complexes in the seminiferous epithelium are involved with internalization of adhesion junctions." in: Biology of reproduction, Vol. 71, Issue 2, pp. 548-59, (2004) (PubMed).

  • Target
    nectin-3 (NECTIN3)
    Andere Bezeichnung
    Nectin-3 (NECTIN3 Produkte)
    Synonyme
    PVRL3, CD113, CDW113, NECTIN-3, PPR3, PRR3, PVRR3, 2610301B19Rik, 3000002N23Rik, 4921513D19Rik, AA407785, AU016832, AW538082, nectin cell adhesion molecule 3, poliovirus receptor-related 3, Nectin3, NECTIN3, PVRL3, nectin3
    Hintergrund
    The monoclonal antibody 103-A1 recognizes mouse nectin-3. Nectin-3 is a 83 kDa type I transmembrane glycoprotein. Nectin, originally isolated as poliovirus receptor-related protein (PRR), is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectins are calcium- independent immunoglobulin-like cell-cell adhesion molecules consisting of four members, nectin 1-4. Nectins homophilically and heterophilically trans-interact to form a variety of cell-cell junctions, including cadherin-based adherens junctions in epithelial cells and fibroblasts in culture, synaptic junctions in neurons, and Sertoli cell-spermatid junctions in testis, in cooperation with, or independently of, cadherins. Both nectin-2 and nectin-3 are ubiquitously expressed, whereas nectin-1 is abundantly expressed in brain. Nectin-2 and -3 are expressed in cells where cadherin is not expressed, such as blood cells and spermatids. All members of the nectin family have two or three splice variants. For nectin-3, three isoforms exist: nectin-3α, -3β and -3γ of which nectin-3α is the largest. Nectin-3, also known as PRR3, is a transmembrane protein that is predominantly expressed in testis and placental tissues as well in many cell lines. Nectin interacts in vivo with both long and short isoforms of afadin, an actin binding protein, at cadherin-based cell-cell adherence junctions in various tissues and cell lines. Furthermore, the ectodomains of nectin-3 and CD155 (Poliovirus Receptor) have shown strong affinity to each other. Injection of antibody 103-A1 into lumen of seminiferous tubules leads to disruption of the actin filaments in Sertoli cells at the Sertoli-maturing spermatid ectoplasmic specialization and exfoliation of maturing spermatids form the seminiferous epithelium. Aliases PPR3, poliovirus receptor-related protein 3, PRR3, PVRR3, CD113 Immunogen Recombinant mouse nectin-3 extracellular domain (50-400 aa) fused to IgG Fc
    Pathways
    Cell-Cell Junction Organization
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