Surface Layer Protein (AA 301-400) Antikörper (Biotin)

Produktnummer ABIN2179505

Der Kaninchen Polyklonal Anti--Antikörper wurde für ELISA, IHC (fro) und IHC (p) validiert. Er ist geeignet, in Proben von Bakterien, Lactobacillus acidophilus, Lactobacillus amylovorus, Lactobacillus crispatus, Lactobacillus gallinarum und Lactobacillus helveticus zu detektieren.

Kurzübersicht für Surface Layer Protein (AA 301-400) Antikörper (Biotin) (ABIN2179505)

Target: Surface Layer Protein

Reaktivität: Bakterien, Lactobacillus acidophilus, Lactobacillus amylovorus, Lactobacillus crispatus, Lactobacillus gallinarum, Lactobacillus helveticus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails

Bindungsspezifität: AA 301-400

Kreuzreaktivität: Bakterien

Kreuzreaktivität (Details): L.acidophilus, L.crispatus, L.helveticus, L.amylovorus, L.gallinarum

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from L, acidophilus S-layer protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC-P 1:200-400
IHC-F 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C for 12 months.

Haltbarkeit: 12 months

Antigendetails

Target: Surface Layer Protein

Hintergrund:

Synonyms: S-layer, Surface layer, surface layer protein, Slp, SLAP_LACAC, S-layer protein, slpA, sl pH , Lactobacillus surface layer protein,

Background: One of the most fascinating cell envelope structures in prokaryotic organisms is two-dimensional arrays of protein or glycoprotein subunits, termed S-layers (Surface Layers). They are composed of numerous identical subunits forming a symmetric, porous, lattice-like layer that completely covers the cell surface. The subunits are held together and attached to cell wall carbohydrates by non-covalent interactions, and they spontaneously reassemble in vitro by an entropy-driven process. In lactobacilli, S-layer proteins have been detected on many but not all species. Lactobacillus S-layer proteins differ from those of other bacteria in their smaller size and high predicted pI. The positive charge in Lactobacillus S-layer proteins is concentrated in the more conserved cell wall binding domain, which can be either N- or C-terminal depending on the species. The more variable domain is responsible for the self-assembly of the monomers to a periodic structure. The biological functions of Lactobacillus S-layer proteins are poorly understood, but in some species S-layer proteins mediate bacterial adherence to host cells or extracellular matrix proteins or have protective or enzymatic functions. Lactobacillus S-layer proteins show potential for use as antigen carriers in live oral vaccine design because of their adhesive and immunomodulatory properties and the general non-pathogenicity of the species.

Gen-ID: 3252774

UniProt: P35829