HPV16 E7 Antikörper (Middle Region)

Produktnummer ABIN2173795

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch HPV16 E7 in WB, IF (p), ELISA, FACS, IF (cc), IHC (p), IHC (fro) und ICC. Er zeigt eine Reaktivität gegenüber Human Papillomavirus 16 (HPV-16) und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für HPV16 E7 Antikörper (Middle Region) (ABIN2173795)

Target: HPV16 E7 (HPV-16 E7) (Human Papilloma Virus 16 E7 (HPV-16 E7))

Reaktivität: Human Papillomavirus 16 (HPV-16)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HPV16 E7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Produktdetails anti-HPV16 E7 Antikörper

Bindungsspezifität: Middle Region

Spezifität: This antibody may also detect HPV35-E7

Kreuzreaktivität: Human, Virus

Kreuzreaktivität (Details): HPV16

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from middle of HPV16-E7

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-HPV16 E7 Antikörper (ABIN2173795)

Liu, Murray-Stewart, Casero, Kagiampakis, Jin, Zhang, Wang, Che, Tong, Ke, Jiang, Wang, Wan: "Targeting hexokinase 2 inhibition promotes radiosensitization in HPV16 E7-induced cervical cancer and suppresses tumor growth." in: International journal of oncology, Vol. 50, Issue 6, pp. 2011-2023, (2017) (PubMed).

Shao, Sun, Wang, Chung, Du, Wang, Qiu, Wang, Wu: "HPV16 E6/E7 upregulates HIF-2α and VEGF by inhibiting LKB1 in lung cancer cells." in: Tumour biology, Vol. 39, Issue 7, pp. 1010428317717140, (2017) (PubMed).

Details zu HPV16 E7

Target: HPV16 E7 (HPV-16 E7) (Human Papilloma Virus 16 E7 (HPV-16 E7))

Substanzklasse: Viral Protein

Hintergrund:

Synonyms: Protein E7, E7

Background: Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Plays also a role in the inhibition of both antiviral and antiproliferative functions of host inteferon alpha.

Gen-ID: 1489079

UniProt: P03129