Rubella Virus Glycoprotein E1 (AA 801-900) Antikörper (AbBy Fluor® 647)

Produktnummer ABIN2173787

Der Kaninchen Polyklonal anti-Rubella Virus Glycoprotein E1 Antikörper wird verwendet zum Nachweis von Rubella Virus Glycoprotein E1 in Proben von Rubella Virus (RuV) und Virus. Er wurde validiert für IF (cc) und IF (p).

Kurzübersicht für Rubella Virus Glycoprotein E1 (AA 801-900) Antikörper (AbBy Fluor® 647) (ABIN2173787)

Target: Rubella Virus Glycoprotein E1

Reaktivität: Rubella Virus (RuV), Virus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: AbBy Fluor® 647

Applikation: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails

Bindungsspezifität: AA 801-900

Kreuzreaktivität: Virus

Kreuzreaktivität (Details): Rubella virus

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Rubella Virus E1 envelope glycoprotein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Rubella Virus Glycoprotein E1

Andere Bezeichnung: E1 glycoprotein

Substanzklasse: Viral Protein

Hintergrund:

Synonyms: E1, E1 envelope glycoprotein, Glycoprotein E1, Spike glycoprotein E1, POLS_RUBVM.

Background: Rubella virus (RV), the sole member of the genus Rubivirus within the family Togaviridae, is a small enveloped, positive strand RNA virus. The nucleocapsid consists of 40S genomic RNA and a single species of capsid protein which is enveloped within a host-derived lipid bilayer containing two viral glycoproteins, E1 (58 kDa) and E2 (42-46 kDa). In virus infected cells, RV matures by budding either at the plasma membrane, or at the internal membranes depending on the cell type and enters adjacent uninfected cells by a membrane fusion process in the endosome, directed by E1-E2 heterodimers. The heterodimer formation is crucial for E1 transport out of the endoplasmic reticulum to the Golgi and plasma membrane. In RV E1, a cysteine at position 82 is crucial for the E1-E2 heterodimer formation and cell surface expression of the two proteins. E1 has been shown to be a type 1 membrane protein, rich in cysteine residues with extensive intramolecular disulphide bonds [PMID: 11682134]