Estrogen Receptor alpha Antikörper (AA 582-595)

Produktnummer ABIN215827

Dieser Maus Monoklonal Antikörper detektiert spezifisch Estrogen Receptor alpha in WB, IHC, ICC, IP und EMSA. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Estrogen Receptor alpha Antikörper (AA 582-595) (ABIN215827)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Estrogen Receptor alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Electrophoretic Mobility-Shift Assay (EMSA)

Klon: C0542

Produktdetails anti-Estrogen Receptor alpha Antikörper

Bindungsspezifität: AA 582-595

Spezifität: Recognizes the human estrogen receptor at ~65kD and ~55kD believed to be the major proteolytic fragment of the estrogen receptor alpha protein. The antibody also recognizes native estrogen receptor in both the inactive 8S oligomeric state, when activated to 4S and whether unbound or bound to ligand. Species cross-reactivity: mouse, rat and hamster.

Homologie: Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon (100%) Gorilla, Monkey (93%) Bat, Bovine, Horse (86%).

Aufreinigung: Protein G purified

Immunogen: A 14 residue C-terminal synthetic peptide, (C)YYITGEAEGFPATV based on the human estrogen receptor (residues 582-595) alpha protein sequence with the cysteine (C) residue added and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon (100%), Gorilla, Monkey (93%), Bat, Bovine, Horse (86%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: GS, ICC, IHC, IP, WB

Usage: Suitable for use in Western Blot, Gel Mobility Shift Assay, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Western Blot (ECL): 2 μg/mL. Gel Mobility Shift Assay: 20 μg/reaction. Immunoprecipitation: 20 μg/100 μL beads. We recommend that protein A Sepharose be used as immunoabsorbant with a bridging rabbit anti-mouse IgG to ensure efficient binding of the antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Andere Bezeichnung: ER Alpha / Estrogen Receptor

Hintergrund: Name/Gene ID: ESR1
Subfamily: NR3 Steroid receptor
Family: NHR

Synonyms: ESR1, Era, ERalpha, Estrogen receptor, Estradiol receptor, ER-alpha, Estrogen receptor 1, NR3A1, ER, ESR, ESRA, Estrogen receptor alpha

Gen-ID: 2099

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly