ATP1A1 Antikörper (AA 338-518)

Produktnummer ABIN215645

Der Kaninchen Polyklonal Anti-ATP1A1-Antikörper wurde für WB, IHC, IP, ICC und IHC (fro) validiert. Er ist geeignet, ATP1A1 in Proben von Ratte und Rind (Kuh) zu detektieren.

Kurzübersicht für ATP1A1 Antikörper (AA 338-518) (ABIN215645)

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Reaktivität: Ratte, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATP1A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-ATP1A1 Antikörper

Bindungsspezifität: AA 338-518

Spezifität: Specific for the a-1 subunit isoform at Mr of 110kD. Species cross-reactivity: Rat and bovine. Does not cross-react with rabbit.

Aufreinigung: Protein G purified

Immunogen: Fusion protein containing residues 338-518 of the a-1 subunit, as deduced from the cDNA.

Type of Immunogen: Fusion protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ICC (5 μg/mL), IHC, IHC-Fr, IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Previous lots were successfully used to immunoprecipitate the a-1 subunit from of rat brain microsomes. Immunocytochemistry: 5 μg/mL detects the Na+ /K+ ATPase a-1 subunit in frozen sections of rat kidney and brain, which were fixed in 4 % paraformaldehyde.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu ATP1A1

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Andere Bezeichnung: ATP1A1

Hintergrund: Name/Gene ID: ATP1A1
Subfamily: ATPase - P type, type IIC
Family: Transporter

Synonyms: ATP1A1, Na+, K+ ATPase alpha subunit, Na,K-ATPase alpha-1 subunit, Na+/K+ ATPase 1, Sodium pump 1, Sodium pump subunit alpha-1

Gen-ID: 476

UniProt: P05023

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Proton Transport, Ribonucleoside Biosynthetic Process