ATP1A1 Antikörper

Produktnummer ABIN215644

Dieses Maus Monoklonal-Antikörper erkennt spezifisch ATP1A1 in WB, IHC und IP. Er zeigt eine Reaktivität gegenüber Human, Ratte, Maus, Schwein, Hund, Affe und Xenopus laevis.

Kurzübersicht für ATP1A1 Antikörper (ABIN215644)

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Reaktivität: Human, Ratte, Maus, Schwein, Hund, Affe, Xenopus laevis

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ATP1A1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Produktdetails anti-ATP1A1 Antikörper

Spezifität: Recognizes rabbit Na+/K+ ATPase alpha-1 subunit. Species cross-reactivity: Human, rabbit, monkey, rat, mouse, lamb, canine, porcine and Xenopus.

Aufreinigung: Protein G purified

Immunogen: Purified Na+/K+ ATPase isolated from membrane fractions of rabbit kidney outer medulla.

Type of Immunogen: Purified protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IP, WB (0.05 - 0.1 μg/mL)

Usage: Western Blot: 0.05-0.1 μg/mL detected the a-1 subunit in 20 μg of rat brain microsomal preparations using chemiluminescence. To reduce background, wash the blot with 0.05 % Tween-20 in PBS for 15 minutes.Immunohistochemistry: 1-10 μg/mL on ice-cold methanol-fixed tissue.Immunoprecipitation: 2 μg immunoprecipitated the a-1 subunit from rat brain microsomal preparations.Optimal dilutions to be determined by the researcher.

Kommentare:

Target Species of Antibody: Rabbit

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Supplied as a liquid in 0.1M Tris-Glycine,  pH 7.4, 0.15M sodium chloride, 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu ATP1A1

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Andere Bezeichnung: ATP1A1

Hintergrund: Name/Gene ID: ATP1A1
Subfamily: ATPase - P type, type IIC
Family: Transporter

Synonyms: ATP1A1, Na+, K+ ATPase alpha subunit, Na,K-ATPase alpha-1 subunit, Na+/K+ ATPase 1, Sodium pump 1, Sodium pump subunit alpha-1

Gen-ID: 476

UniProt: P05023

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Proton Transport, Ribonucleoside Biosynthetic Process