TCP1 alpha/CCTA Antikörper (C-Term)

Produktnummer ABIN215497

Dieses Ratte Monoklonal-Antikörper erkennt spezifisch TCP1 alpha/CCTA in WB, IP und ICC. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh), Hund, Kaninchen, Hamster, Affe, Meerschweinchen, Schwein und Drosophila melanogaster.

Kurzübersicht für TCP1 alpha/CCTA Antikörper (C-Term) (ABIN215497)

Target: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Kaninchen, Hamster, Affe, Meerschweinchen, Schwein, Drosophila melanogaster

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser TCP1 alpha/CCTA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-TCP1 alpha/CCTA Antikörper

Bindungsspezifität: C-Term

Spezifität: This affinity purified antibody recognizes an epitope in the C-terminus of mouse TCP-1a (465-AKLRA) and will detect an ~60kD protein, corresponding to the apparent molecular mass of TCP-1alpha on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, guinea pig, hamster, monkey, porcine and rabbit origins, as well as Saccharomyces cerevisiae (weakly, consistent with the epitope sequence being AKLRS), Drosophila, Schizosaccharomyces pombe and C. elegans. In C. elegans CTA-191 reacts with TCP-1a and another CCT subunit. In plants, CTA-191 recognizes TCP-1 of Pisum sativum and the sequence of Arabidopsis thalania TCP-1a over the region of the epitope AKLRA. It has also been shown that CTA-191 reacts with a subunit of a specialized chaperonin which folds phytochrome. Cross reactivity with human Hsp60 has been observed with this antibody in immunoblot analysis.

Aufreinigung: Protein G purified

Immunogen: A purified recombinant mouse TCP-1alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp-1b gene expressed in E. coli.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: ICC (5 μg/mL), IP (12.5 μg/mL), WB

Usage: Western Blot (Colorimetric): 1 μg/mL. Immunoprecipitation: 12.5 μg/mL 5 μg/mL. Immunocytochemistry: 5 μg/mL. Positive control: HeLa Heat Shocked Cell Lysate.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 mM PMSF, in 50 % glycerol.

Konservierungsmittel: PMSF

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu TCP1 alpha/CCTA

Target: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Andere Bezeichnung: TCP1

Hintergrund: Name/Gene ID: TCP1

Synonyms: TCP1, CCTA, CCT-alpha, D6S230E, T-complex 1, TCP-1-alpha, Tailless complex polypeptide 1, CCT1

Gen-ID: 6950

UniProt: P17987