CD4 Antikörper (APC)

Produktnummer ABIN2145315

Produktdetails anti-CD4 Antikörper (APC)

Target: CD4

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD4 Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS)

Produktmerkmale: The 4AGK1.5 monoclonal antibody reacts with the mouse CD4 Molecule, a 55 kDa cell surface receptor. It is a member of the lg superfamily,primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC classII and associating with the protein tyrosine kinase, lck.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdurchführung:

• Take 100 μL a certain amount of prepared cells and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.3-0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined in two hours on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu CD4

Target: CD4

Andere Bezeichnung: CD4 (CD4 Produkte)

Pathways: T-Zell Rezeptor Signalweg, Maintenance of Protein Location, CXCR4-mediated Signaling Events