CD37 Antikörper (PE)

Produktnummer ABIN2144866

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD37 in FACS, ELISA, IF und IHC (p). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CD37 Antikörper (PE) (ABIN2144866)

Target: CD37 (TSPAN26) (Tetraspanin 26 (TSPAN26))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD37 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 4AIPO-24

Produktdetails anti-CD37 Antikörper (PE)

Produktmerkmale: 4AIPO-24 reacts with CD37 (a.k.a. gp52-40 ), a 40-52 kDa molecule, which is strongly expressed on B cells from the pre-B cell stage, but not on plasma cells. It is also present at low levels on some T cells, monocytes and granulocytes. CD37 is a stable marker for malignancies derived from mature B cells, such as B-CLL, HCL and all types of B-NHL. CD37 is involved in signal transduction.CD37 is stable marker for malignancies derived from mature B cells: B-CLL. HCL and B-NHL.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdurchführung:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu CD37

Target: CD37 (TSPAN26) (Tetraspanin 26 (TSPAN26))

Andere Bezeichnung: CD37

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response