CD19 Antikörper (PE)

Produktnummer ABIN2144814

Dieses Anti-CD19-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD19 in FACS. Geeignet für Human.

Kurzübersicht für CD19 Antikörper (PE) (ABIN2144814)

Target: CD19 (CD19 Molecule (CD19))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD19 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Klon: 4ACB19

Produktdetails anti-CD19 Antikörper (PE)

Produktmerkmale: 4ACB19 reacts with CD19 (B4), a 90 kDa molecule, which is expressed on approximately 5-25 % of human peripheral blood lymphocytes. CD19 antigen is present on human B lymphocytes at most stages of maturation, from the earliest Ig gene rearrangement in pro-B cells to mature cell, as well as malignant B cells, but is lost on maturation to plasma cells. CD19 antibody does not react with T lymphocytes, monocytes and granulocytes. CD19 is a critical signal transduction molecule that regulates B lymphocyte development, activation and differentiation. This clone is cross reactive with non-human primate.* CD19 is a key phenotyping marker of non-T cell leukemia.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdurchführung:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu CD19

Target: CD19 (CD19 Molecule (CD19))

Andere Bezeichnung: CD19

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung