HMGN1 Antikörper (pSer20, Ser24)

Produktnummer ABIN214463

Der Kaninchen Polyklonal Anti-HMGN1 Antikörper wurde für WB, IHC, ELISA und IHC (p) validiert. Er ist geeignet, HMGN1 in Proben von Human zu detektieren.

Kurzübersicht für HMGN1 Antikörper (pSer20, Ser24) (ABIN214463)

Target: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HMGN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-HMGN1 Antikörper

Bindungsspezifität: pSer20, Ser24

Spezifität: Amino acids 19-28 of human HMGN protein containing pSer20 and pSer24

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from human HMGN1.

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:5000 - 1:25000), IHC, IHC-P (20 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu HMGN1

Target: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Andere Bezeichnung: HMGN1

Hintergrund: Name/Gene ID: HMGN1

Synonyms: HMGN1, HMG14

Gen-ID: 3150

UniProt: P05114

Pathways: Chromatin Binding