CD16 Antikörper (FITC)

Produktnummer ABIN2144518

Produktdetails anti-CD16 Antikörper (FITC)

Target: CD16

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD16 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Produktmerkmale: 4ACB16 reacts with human CD16, a 50-65 kDa Fcg receptor IIIa (FcgRIII), expressed on NK cells, monocytes/macrophages and granulocytes. It is a human NK cell associated antigen. CD16 is a low affinity receptor for IgG which functions in phagocytosis and ADCC, as well as in signal transduction and NK cell activation. The CD16 antibody blocks the binding of soluble immune complexes to granulocytes.This clone is cross reactive with non-human primate.

Klon: 4ACB16

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdurchführung:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu CD16

Target: CD16

Andere Bezeichnung: CD16 (CD16 Produkte)

Synonyme: Fcgr3 antikoerper, Fcgr3a antikoerper, CD16 antikoerper, FCGR3 antikoerper, FCGR3@ antikoerper, CD16b antikoerper, FCG3 antikoerper, FCR-10 antikoerper, FCRIII antikoerper, FCRIIIb antikoerper, FcRIII antikoerper, Fc fragment of IgG, low affinity IIa, receptor antikoerper, Fc fragment of IgG, low affinity IIIa, receptor (CD16a) antikoerper, Fc fragment of IgG receptor IIIb antikoerper, Fc fragment of IgG receptor IIIa antikoerper, Fc receptor, IgG, low affinity III antikoerper, Fc-gamma RIII antikoerper, Fcgr2a antikoerper, FCGR3A antikoerper, FCGR3B antikoerper, Fcgr3 antikoerper, FCGR3 antikoerper

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process