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CD5 Antikörper (CD5 Molecule) (FITC) Primary Antibody

CD5 Reaktivität: Human CyTOF, FACS, IHC, WB Wirt: Maus Monoclonal 4AUCHT2
Produktnummer ABIN2144467
$248.16
Zzgl. Versandkosten $45.00
50 tests
local_shipping Lieferung nach: Vereinigte Staaten von Amerika
Lieferung in 12 bis 14 Werktagen
  • Target
    Reaktivität
    Human
    Wirt
    Maus
    Klonalität
    Monoklonal
    Konjugat
    Dieser CD5 Antikörper ist konjugiert mit FITC
    Applikation
    Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
    Produktmerkmale
    4AUCHT2 reacts with 58 kDa molecule, a member of the scavenger receptor superfamily, expressed on thymocytes and all mature T lymphocytes. It also expressed on a small subset of mature B lymphocytes ( B1a cells ) which is expanded during fetal life, and in several autoimmune disorders, as well as in some B-CLL .CD5 may serve as a dual receptor which provides inhibitiry signals in thymocytes and B1a cells and acts as a costimulatory signal receptor. CD5-mediated cellular interaction may influence thymocyte maturation and selection. CD5 is a phenotypic marker for some B-cell lymphoproliferative disorders (B-CLL, mantle zone lymphoma , hairy cell leukemia, etc). The increase of blood CD3+/CD5- T cells correlates with the presence of GVHD.
    Klon
    4AUCHT2
    Isotyp
    IgG1
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Testdurchführung
    • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
    • Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
    • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
    • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
    • [Cell fixation: 2 % formaldehyde solution]
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
  • Target
    Andere Bezeichnung
    CD5 (CD5 Antibody Abstract)
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