S100 Protein (S100) Antikörper

Produktnummer ABIN214427

Der Kaninchen Polyklonal anti-S100 Protein Antikörper wird verwendet zum Nachweis von S100 Protein in Proben von Human und Rind (Kuh). Er wurde validiert für WB, IHC, ELISA und IHC (p).

Kurzübersicht für S100 Protein (S100) Antikörper (ABIN214427)

Target: S100 Protein (S100)

Reaktivität: Human, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails

Spezifität: S100 protein

Aufreinigung: Protein A purified

Immunogen: Full-length bovine S100 protein (mixture of aa homodimers and ab heterodimers).

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:5000 - 1:20000), IHC, IHC-P (2.5 μg/mL), WB (1:500 - 1:3000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Bovine

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Antigendetails

Target: S100 Protein (S100)

Andere Bezeichnung: S100 Protein

Pathways: Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades, S100 Proteine