SAE1 Antikörper

Produktnummer ABIN214379

Der Kaninchen Polyklonal anti-SAE1 Antikörper wird verwendet zum Nachweis von SAE1 in Proben von Human. Er wurde validiert für WB, ELISA, IF, IHC und IHC (p).

Kurzübersicht für SAE1 Antikörper (ABIN214379)

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SAE1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SAE1 Antikörper

Spezifität: Recombinant protein produced by baculoviral expression in insect cells (Sf9, Spodoptera frugiperda) corresponding to full length Human SUMO Activating Enzyme E1 fused with GST.

Aufreinigung: Protein A purified

Immunogen: Recombinant human SAE1.

Type of Immunogen: Recombinant protein

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:5000 - 1:20000), IF, IHC, IHC-P (5 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: After adding water, will consist of 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2 with 0.01 % (w/v) Sodium Azide

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu SAE1

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Andere Bezeichnung: SAE1

Hintergrund: Name/Gene ID: SAE1

Synonyms: SAE1, Activator of SUMO1, AOS1, UBLE1A, HSPC140, SUA1

Gen-ID: 10055

UniProt: Q9UBE0