Fibronectin Antikörper

Produktnummer ABIN2141941

Der Kaninchen Polyklonal anti-Fibronectin Antikörper wird verwendet zum Nachweis von Fibronectin in Proben von Human. Er wurde validiert für WB, IHC, ELISA und IP.

Kurzübersicht für Fibronectin Antikörper (ABIN2141941)

Target: Fibronectin

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Fibronectin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Produktdetails anti-Fibronectin Antikörper

Spezifität: This antibody is specific for Human Factor XIII.

Kreuzreaktivität (Details): This antibody reacts with Human Fibronectin. Ths antibody has negligible cross reactivity with Type I,II,III,IV,V,VI Collagens or Laminin1.0 mg/ml

Aufreinigung: Affinity chromatography purified

Sterilität: Sterile filtered

Immunogen: Fibronectin antibody was raised in rabbit using fibronectin purified from human plasma as the immunogen.

Anwendungsinformationen

Applikationshinweise: ELISA: 1:5,000-1:10,000, IHC: 1:50-1:200, IP: 1:100, WB: 1:5,000-1:10,000
Optimal conditions should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Sterile filtered liquid In 0.02 M K3PO4, pH 7.2, with 0.15 M NaCl, and 0.01 % Sodium azide.

Vorsichtsmaßnahmen: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Details zu Fibronectin

Target: Fibronectin

Hintergrund: Human fibronectin has a molecular weight of 450,000 daltons when purified in an intact form from plasma. Fibronectin is a glycoprotein synthesized in the liver for the circulating blood plasma form, and is synthesized by many mesenchymal cells, for the extracellular matrix form. It is composed of two similar, but not identical protein chains, which are linked by two disulfide linkages at the C-terminal end of the chains. The chains are composed of domains which have specific secondary structures linked together by regions which are especially susceptible to proteolytic action. For this reason, detection by immunoblot (western) may show considerable variability in the observed apparent molecular weights, which is predicated on the source of the fibronectin, and to what degree proteolysis has occurred. Bands approximately 225 kDa should be observed after SDS-PAGE when reducing and denaturing conditions are used.