CHRM3 Antikörper (Cytoplasmic Domain)

Produktnummer ABIN213346

Produktdetails anti-CHRM3 Antikörper

Target: CHRM3 (Cholinergic Receptor, Muscarinic 3 (CHRM3))

Bindungsspezifität: Cytoplasmic Domain

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHRM3 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human CHRM3 / M3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%) Orangutan, Sheep, Bovine, Hamster, Horse (94%) Elephant, Pig (89%) Mouse, Rat, Dog, Rabbit, Opossum, Guinea pig, Turkey, Chicken, Lizard (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 18 amino acid peptide from 3rd cytoplasmic domain of human CHRM3 / M3. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%), Orangutan, Sheep, Bovine, Hamster, Horse (94%), Elephant, Pig (89%), Mouse, Rat, Dog, Rabbit, Opossum, Guinea pig, Turkey, Chicken, Lizard (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu CHRM3

Target: CHRM3 (Cholinergic Receptor, Muscarinic 3 (CHRM3))

Andere Bezeichnung: CHRM3 / M3 (CHRM3 Produkte)

Hintergrund: Name/Gene ID: CHRM3
Subfamily: Acetylcholine
Family: GPCR

Synonyms: CHRM3, M3, Muscarinic receptor M3, Muscarinic M3 receptor, EGBRS, HM3, M3 muscarinic receptor

Gen-ID: 1131

Pathways: Synaptic Membrane