PML Antikörper (AA 1-581)

Produktnummer ABIN212004

Dieses Anti-PML-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von PML in WB und ICC. Geeignet für Maus.

Kurzübersicht für PML Antikörper (AA 1-581) (ABIN212004)

Target: PML (Promyelocytic Leukemia (PML))

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PML Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC)

Produktdetails anti-PML Antikörper

Bindungsspezifität: AA 1-581

Spezifität: Recognizes mouse PML, Mr ~90-106kD, depending on degree of protein modification. Does not cross-react with human. MEF1 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with This antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

Aufreinigung: Protein G purified

Immunogen: 6His fusion protein corresponding to aa1-581 of mouse PML (promyelocytic leukemia protein).

Type of Immunogen: Fusion protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Approved: ICC, WB (0.5 - 1 μg/mL)

Usage: Suitable for use in Western Blot and Immunocytochemistry. Western Blot: 0.5-1 μg/mL of this lot detected PML in RIPA lysates from mouse embryo fibroblast (MEF1) cells. Immunocytochemistry: An independent laboratory showed positive immunostaining for PML in MEF1 cells fixed with 4 % paraformaldehyde and permeabilized with 0.2 % Triton X-100.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu PML

Target: PML (Promyelocytic Leukemia (PML))

Andere Bezeichnung: PML

Hintergrund: Name/Gene ID: PML
Family: Transcription factor

Synonyms: PML, PP8675, Promyelocytic leukemia, Protein PML, TRIM19, Promyelocytic leukemia protein, RING finger protein 71, MYL, RNF71

Gen-ID: 5371

UniProt: P29590

Pathways: p53 Signalweg, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus