LCK Antikörper (AA 1-58)

Produktnummer ABIN211979

Der Kaninchen Polyklonal Anti-LCK-Antikörper wurde für WB und IP validiert. Er ist geeignet, LCK in Proben von Human, Maus und Rind (Kuh) zu detektieren.

Kurzübersicht für LCK Antikörper (AA 1-58) (ABIN211979)

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Reaktivität: Human, Maus, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LCK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-LCK Antikörper

Bindungsspezifität: AA 1-58

Spezifität: Recognizes Lck, Mr 56kD of mouse, human and bovine, reactivity with other Src family members not determined.

Aufreinigung: Protein A purified

Immunogen: GST fusion protein corresponding to amino acids 1-58 of human Lck.

Type of Immunogen: Fusion protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IP, WB

Usage: Western Blot Analysis: 0.5-2 μg/mL detected Lck in RIPA lysates from Jurkat cells. Included Positive Antigen Control: Jurkat cell lysate. Use 20 μg per lane for minigels. Immunoprecipitation: 4 μg immunoprecipitated Lck from 1 μg of Jurkat RIPA lysate as confirmed by subsequent immunoblot using monoclonal anti-Lck. Western Blot Analysis Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Lck (1 μg/mL). Proteins were visualized using a goat anti rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu LCK

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Andere Bezeichnung: p56lck / LCK

Hintergrund: Name/Gene ID: LCK
Subfamily: Src
Family: Protein Kinase

Synonyms: LCK, Lck kinase, LSK, p56-LCK, Pp58lck, Tyrosine-protein kinase Lck, p56lck, Protein YT16, Proto-oncogene Lck, YT16

Gen-ID: 3932

UniProt: P06239

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling