CD58 Antikörper (Cytoplasmic Domain)

Produktnummer ABIN211812

Produktdetails anti-CD58 Antikörper

Target: CD58

Bindungsspezifität: Cytoplasmic Domain

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD58 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB)

Spezifität: CD58, or LFA-3, is a membrane glycoprotein of 55-70kD. It occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA. It is heavily N-glycosylated. CD58 is a cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes[1]. It is a member of the immunoglobulin superfamily of molecules. CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells[2]. There are approximately 5000 CD58 Molecules on each erythrocyte. There is reduced expression of CD58 on hemopoietic cells in individuals with paroxysmal nocturnal hemoglobinuria. BRIC5 was produced in response to erythrocytes. The functional affinity of BRIC5 binding to erythrocytes is 4 x 108 M-1. Reacts by immunoblotting to non-reduced erythrocyte membranes. BRIC5 is an indirect hemagglutinin. The antigen on erythrocytes is Pronase sensitive. BRIC5 inhibits T cell rosetting.

Aufreinigung: Protein G purified

Immunogen: Human erythrocytes.

Type of Immunogen: Cells

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: Flo (1:10), WB

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request.

Not recommended for: IHC-Fr, IHC-P

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: deionized water

Konzentration: Lot specific

Buffer: 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu CD58

Target: CD58

Andere Bezeichnung: CD58 (CD58 Produkte)

Hintergrund: Name/Gene ID: CD58
Family: Immunoglobulin

Synonyms: CD58, CD58 antigen, AG3, LFA-3, LFA3, Surface glycoprotein LFA-3, Surface glycoprotein lfa3, CD58 Molecule

Gen-ID: 965