Histone H2B Antikörper (AA 118-126)

Produktnummer ABIN211182

Dieses Anti-Histone H2B-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Histone H2B in WB. Geeignet für Human.

Kurzübersicht für Histone H2B Antikörper (AA 118-126) (ABIN211182)

Target: Histone H2B

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone H2B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Histone H2B Antikörper

Bindungsspezifität: AA 118-126

Spezifität: Recognizes and is specific for Histone H2B, Mr ~15kD. An additional band at 23kD is detected in some samples, which likely corresponds to ubiquityl-Histone H2B. Species cross-reactivity: Human, chicken and Xenopus. Others not tested. Broad species cross-reactivity expected based on sequence homology.

Homologie: Percent identity by BLAST analysis: Human, Orangutan, Mouse, Rat, Bovine, Chicken, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito, Nematode (90%).

Aufreinigung: Protein A purified

Immunogen: KLH-conjugated, synthetic peptide corresponding to amino acids 118-126 (CGAVTKYTSSK) of human Histone H2B, with an N-terminal CG added for conjugation purposes. Percent identity by BLAST analysis: Human, Orangutan, Mouse, Rat, Bovine, Chicken, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito, Nematode (90%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: WB (1:1000 - 1:10000)

Usage: Suitable for use in Western Blot. Western Blot: 1:1000-1:10000 detected Histone H2B in acid extracts from either sodium butyrate or colcemid treated HeLa cells and 13-114 respectively), chicken core histone and recombinant Xenopus Histone H2B.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 200 μLof 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu Histone H2B

Target: Histone H2B