Histone H1 Antikörper (hyperphosphorylated)
Kurzübersicht für Histone H1 Antikörper (hyperphosphorylated) (ABIN211181)
Target
Alle Histone H1 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- hyperphosphorylated
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Spezifität
- Recognizes phosphorylated histone H1 (32kD). Additional proteins (Mr 66 and 100kD) were detected. Species cross-reactivity: human, bovine.
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Aufreinigung
- Protein A purified
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Immunogen
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Hyper-phosphorylated isoform of Tetrahymena Histone H1.
Type of Immunogen: Purified protein -
Isotyp
- IgG
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Applikationshinweise
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Approved: ICC (5 - 10 μg/mL), WB (1 - 2 μg/mL)
Usage: Suitable for use in Western Blot and Immunocytochemistry. Proteins were visualized using a goat anti-rabbit secondary antibody labeled with HRP. A chemiluminescence detection system was used. Immunocytochemistry: 5-10 μg/mL showed positive immunostaining for Histone H1 in mitotic HeLa cells fixed with 95 % ethanol, 5 % glacial acetic acid. -
Kommentare
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Target Species of Antibody: Human
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- Lot specific
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Buffer
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Avoid repeat freeze-thaw cycles.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Histone H1
Target
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