CD300a Antikörper

Produktnummer ABIN206697

Dieser Anti-CD300a Antikörper ist ein Maus Monoklonal Antikörper zur Detektion von CD300a in WB und FACS. Geeignet für Human.

Kurzübersicht für CD300a Antikörper (ABIN206697)

Target: CD300a (CD300A)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD300a Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Klon: MEM-260

Produktdetails anti-CD300a Antikörper

Spezifität: Recognizes human CD300a, otherwise known as IRp60 (inhibitory receptor protein 60), a 60kD transmembrane glycoprotein expressed by natural killer cells (NK), T and B cell subsets, monocytes, neutrophils, macrophages, granulocytes, dendritic cells (DC) and mast cells. Studies have shown that the cross-linking of CD300a on NK results in a down-regulation of their cytolytic activity, whilst cross-linking of CD300a on mast cells, inhibits IgE-induced degranulation and SCF-mediated survival. Furthermore, cross-linking of CD300a on the surface of eosinophils has been shown to significantly inhibit their survival, chemotaxis and activation in response to certain inflammatory cytokines, whilst eosinophil-derived MBP (major basic protein) and EDN (neurotoxin), can down-regulate CD300a expression on mast cells in vitro.

Aufreinigung: Protein A purified

Immunogen: Human HPB acute lymphoid leukaemia cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: Flo, WB

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Method sheets are available on request. Western Blot: LS-C45034 detects a band of approximately 60kD in HPB cell lysates under non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu CD300a

Target: CD300a (CD300A)

Andere Bezeichnung: CD300A

Hintergrund: Name/Gene ID: CD300A

Synonyms: CD300A, CD300a antigen, CD300a molecule, CMRF35-like molecule 8, CMRF35-H, CMRF35-H9, CLM-8, CMRF35H9, IGSF12, Inhibitory receptor protein 60, IRC2, IRC1, NK inhibitory receptor, CMRF-35-H9, CMRF-35H, CMRF35H, IRC1a, IRp60, Leukocyte membrane antigen

Gen-ID: 11314

UniProt: Q9UGN4

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process