Nestin Antikörper

Produktnummer ABIN205661

Der Maus Monoklonal Anti-Nestin-Antikörper wurde für WB, IHC und IHC (fro) validiert. Er ist geeignet, Nestin in Proben von Maus und Ratte zu detektieren.

Kurzübersicht für Nestin Antikörper (ABIN205661)

Target: Nestin (NES)

Reaktivität: Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nestin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: Rat-401-4D4

Produktdetails anti-Nestin Antikörper

Spezifität: Recognizes rat nestin, a large intermediate filament protein transiently expressed in embryonic glial cells. It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas.

Keine Kreuzreaktivität: Human

Aufreinigung: Purified

Immunogen: Nestin purified from embryonic rat spinal cord.

Type of Immunogen: Purified protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-Fr (1:20 - 1:200), WB

Usage: Immunohistochemistry: We recommend perfusing tissues with 4 % paraformaldehyde at  pH 7.4 for light microscopy or with either 4 % paraformaldehyde at  pH 10.0 or 4 % paraformaldehyde with 0.1 % glutaraldehyde at  pH 7.4 for EM. For Immunocytochemistry we recommend using cells fixed in 4 % paraformaldehyde buffered with 50 mM sodium borate at  pH 9.5. Western Blot: LS-C42054 reacts with a band at 200-220 kD in reducing gels of newborn rat or mouse cell extracts. For the purpose of western blotting it is recommended that samples should be boiled in 4 volumes of 125 mM Tris,  pH 6.8, 10 % 2-mercaptoethanol, 10 % glycerol and 4.6 % SDS. Membranes should be blocked with milk or BSA. 5 % PAGE gels are suggested.

Not recommended for: IHC-P

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles. Store undiluted.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Details zu Nestin

Target: Nestin (NES)

Andere Bezeichnung: Nestin

Hintergrund: Name/Gene ID: NES

Synonyms: NES, Nestin, Nbla00170

Gen-ID: 10763