Occludin Antikörper (C-Term) (FITC)

Produktnummer ABIN204503

Der Maus Monoklonal Anti-Occludin-Antikörper wurde für WB, ELISA und IF validiert. Er ist geeignet, Occludin in Proben von Human, Maus, Ratte und Hund zu detektieren.

Kurzübersicht für Occludin Antikörper (C-Term) (FITC) (ABIN204503)

Target: Occludin (OCLN)

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Occludin Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Klon: OC-3F10

Produktdetails anti-Occludin Antikörper (FITC)

Bindungsspezifität: C-Term

Spezifität: Recognizes human Occludin Protein. Species cross-reactivity: mouse, rat and canine.

Aufreinigung: Protein A purified

Immunogen: GST fusion protein consisting of the C-terminal region (~150aa) of human occludin.

Type of Immunogen: Fusion protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.1 - 1 μg/mL), IF (2 - 10 μg/mL), WB (0.1 - 1 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunofluorescence. ELISA: 0.1-1 μg/mL. Western Blot: Detects a band at ~65kD. Immunofluorescence: 2-10 μg/mL. Positive control: T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), Caco-2 cells (human colon adenocarcinoma) and rat liver. Does not appear to detect the most highly phosphorylated form of the occludin protein when tested on Caco-2 cells. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide, 1 % BSA, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: -20°C
Protect from light.

Details zu Occludin

Target: Occludin (OCLN)

Andere Bezeichnung: OCLN / Occludin

Hintergrund: Name/Gene ID: OCLN

Synonyms: OCLN, Occludin, BLCPMG

Gen-ID: 100506658

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C