RFXANK Antikörper

Produktnummer ABIN204403

Dieser Maus Monoklonal Antikörper detektiert spezifisch RFXANK in WB, ELISA, IF, IHC, IP und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Hund und Kaninchen.

Kurzübersicht für RFXANK Antikörper (ABIN204403)

Target: RFXANK (Regulatory Factor X-Associated Ankyrin Containing Protein (RFXANK))

Reaktivität: Human, Maus, Ratte, Hund, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RFXANK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-RFXANK Antikörper

Spezifität: Specific for s all splice forms of Ankyrin G containing a spectrin-binding domain. Does not cross-react with related ankyrin isoforms. Species cross-reactivity: Human, mouse, rabbit, rat, canine.

Aufreinigung: Protein A purified

Immunogen: Synthetic peptide derived from the spectrin-binding domain of the human ankyrin G protein.

Type of Immunogen: Synthetic peptide

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.1 - 1 μg/mL), IF, IHC, IHC-Fr, IP, WB (1 - 3 μg/mL)

Usage: Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. ELISA (native): 0.1-1.0 μg/mL. Detects a band between ~200-300kD. Western Blot: 1-3 μg/mL. For best results on rat brain, mouse brain or MDCK cell lysates, add 0.3 M sucrose to incubation buffer. Immunofluorescence (frozen sections): 5-10 μg/mL. Sensitive to fixation conditions. Recommend 1 % paraformaldehyde for perfusion and 4 hrs of post fixation, after perfusion. For sciatic nerve, recommend 2 % paraformaldehyde for 2 hrs at 4?C. Immunoprecipitation (native): 5 μg/ IP.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu RFXANK

Target: RFXANK (Regulatory Factor X-Associated Ankyrin Containing Protein (RFXANK))

Andere Bezeichnung: RFXANK

Hintergrund: Name/Gene ID: RFXANK

Synonyms: RFXANK, ANKRA1, BLS, DNA-binding protein RFXANK, F14150_1, RFXB, RFX-B, Regulatory factor X subunit B, RFX-Bdelta4

Gen-ID: 8625

UniProt: O14593