ESAM Antikörper
Kurzübersicht für ESAM Antikörper (ABIN204196)
Target
Alle ESAM Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Spezifität
- Selected for its ability to detect human ESAM in direct ELISAs and Western Blots. In these applications, this antibody shows no cross-reactivity with rmESAM.
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Aufreinigung
- Protein G purified
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Immunogen
- NS0-derived rhESAM.
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Isotyp
- IgG2b
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Applikationshinweise
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Approved: ELISA, Flo, ICC, WB
Usage: Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect human ESAM. Using a colorimetric detection system, the detection limit for rhESAM is approximately 25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry: This antibody was validated for flow cytometry using HUVECs. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. HUVECs were stained with anti-ESAM or isotype control antibody, followed by PE-conjugated anti-mouse antibody. Immunocytochemistry: This antibody was used at a concentration of 10 μg/mL to detect ESAM in HUVECs. Cells were fixed with PBS containing 4 % paraformaldehyde and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA. After blocking, cells were incubated with diluted primary antibody followed by NL557-coupled anti-mouse IgG in the dark. Between each step, cells were washed with PBS containing BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human ESAM. The detection limit for rhESAM is approximately 1 ng/well. -
Kommentare
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Target Species of Antibody: Human
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- deionized water
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Konzentration
- Lot specific
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Buffer
- Lyophilized.
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- ESAM (Endothelial Cell Adhesion Molecule (ESAM))
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Andere Bezeichnung
- ESAM
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Hintergrund
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Name/Gene ID: ESAM
Synonyms: ESAM, LP4791 protein, W117m -
Gen-ID
- 90952
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UniProt
- Q96AP7
Target
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