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ESAM Antikörper

Dieses Maus Monoklonal-Antikörper erkennt spezifisch ESAM in WB, ELISA, ICC und FACS. Er zeigt eine Reaktivität gegenüber Human.
Produktnummer ABIN204196

Kurzübersicht für ESAM Antikörper (ABIN204196)

Target

Alle ESAM Antikörper anzeigen
ESAM (Endothelial Cell Adhesion Molecule (ESAM))

Reaktivität

  • 43
  • 28
  • 18
Human

Wirt

  • 60
  • 9
  • 5
Maus

Klonalität

  • 62
  • 12
Monoklonal

Konjugat

  • 34
  • 11
  • 10
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser ESAM Antikörper ist unkonjugiert

Applikation

  • 63
  • 26
  • 23
  • 17
  • 13
  • 13
  • 12
  • 7
  • 5
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)
  • Spezifität

    Selected for its ability to detect human ESAM in direct ELISAs and Western Blots. In these applications, this antibody shows no cross-reactivity with rmESAM.

    Aufreinigung

    Protein G purified

    Immunogen

    NS0-derived rhESAM.

    Isotyp

    IgG2b
  • Applikationshinweise

    Approved: ELISA, Flo, ICC, WB

    Usage: Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect human ESAM. Using a colorimetric detection system, the detection limit for rhESAM is approximately 25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry: This antibody was validated for flow cytometry using HUVECs. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. HUVECs were stained with anti-ESAM or isotype control antibody, followed by PE-conjugated anti-mouse antibody. Immunocytochemistry: This antibody was used at a concentration of 10 μg/mL to detect ESAM in HUVECs. Cells were fixed with PBS containing 4 % paraformaldehyde and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA. After blocking, cells were incubated with diluted primary antibody followed by NL557-coupled anti-mouse IgG in the dark. Between each step, cells were washed with PBS containing BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human ESAM. The detection limit for rhESAM is approximately 1 ng/well.

    Kommentare

    Target Species of Antibody: Human

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    deionized water

    Konzentration

    Lot specific

    Buffer

    Lyophilized.
  • Target

    ESAM (Endothelial Cell Adhesion Molecule (ESAM))

    Andere Bezeichnung

    ESAM

    Hintergrund

    Name/Gene ID: ESAM

    Synonyms: ESAM, LP4791 protein, W117m

    Gen-ID

    90952

    UniProt

    Q96AP7
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