ESAM Antikörper

Produktnummer ABIN204196

Dieser Maus Monoklonal Antikörper erkennt spezifisch ESAM in WB, ELISA, ICC und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für ESAM Antikörper (ABIN204196)

Target: ESAM (Endothelial Cell Adhesion Molecule (ESAM))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ESAM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-ESAM Antikörper

Spezifität: Selected for its ability to detect human ESAM in direct ELISAs and Western Blots. In these applications, this antibody shows no cross-reactivity with rmESAM.

Aufreinigung: Protein G purified

Immunogen: NS0-derived rhESAM.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo, ICC, WB

Usage: Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect human ESAM. Using a colorimetric detection system, the detection limit for rhESAM is approximately 25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry: This antibody was validated for flow cytometry using HUVECs. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. HUVECs were stained with anti-ESAM or isotype control antibody, followed by PE-conjugated anti-mouse antibody. Immunocytochemistry: This antibody was used at a concentration of 10 μg/mL to detect ESAM in HUVECs. Cells were fixed with PBS containing 4 % paraformaldehyde and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA. After blocking, cells were incubated with diluted primary antibody followed by NL557-coupled anti-mouse IgG in the dark. Between each step, cells were washed with PBS containing BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human ESAM. The detection limit for rhESAM is approximately 1 ng/well.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: deionized water

Konzentration: Lot specific

Buffer: Lyophilized.

Details zu ESAM

Target: ESAM (Endothelial Cell Adhesion Molecule (ESAM))

Andere Bezeichnung: ESAM

Hintergrund: Name/Gene ID: ESAM

Synonyms: ESAM, LP4791 protein, W117m

Gen-ID: 90952

UniProt: Q96AP7