Aktivieren Sie Javascript in Ihrem Browser, um unsere Webseite optimal zu nutzen.

CD58 Antikörper (Biotin)

CD58 Reaktivität: Human FACS, IHC, IP, IHC (fro), CyTox Wirt: Maus Monoclonal Biotin

Produktnummer ABIN204009

Datenblatt (PDF)

Target Alle CD58 Antikörper anzeigen

CD58
Reaktivität
78

5

3

2

1
Human
Wirt
43

35
Maus
Passende Sekundärantikörper
Klonalität
42

36
Monoklonal
Konjugat
31

6

6

5

4

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser CD58 Antikörper ist konjugiert mit Biotin
Applikation
40

34

13

13

10

8

7

5

5

3

3

2

1

1
Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox)

Spezifität

Reacts with both transmembrane and GPI anchored CD58. Recognizes the CD58 glycoprotein of about 60-70kD and will inhibit HLA-DR mediated T cell cytotoxicity.

Aufreinigung

Protein A purified

Immunogen

Human cytotoxic T cells.

Isotyp

IgG1

Top Product

Discover our top product CD58 Primärantikörper
anti-CD58 (CD58) (AA 1-240) antibody
CD58 Reaktivität: Human WB, ELISA, IHC (p), IF Wirt: Maus Monoclonal 2D11-B10 unconjugated

anti-CD58 (CD58) (AA 1-214) antibody
CD58 Reaktivität: Human WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-CD58 (CD58) antibody
CD58 Reaktivität: Human, Schwein FACS Wirt: Maus Monoclonal MEM-63 unconjugated

anti-CD58 (CD58) (AA 40-220) antibody
CD58 Reaktivität: Human WB, ELISA, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-CD58 (CD58) antibody (Biotin)
CD58 Reaktivität: Human, Schwein FACS Wirt: Maus Monoclonal MEM-63 Biotin

anti-CD58 (CD58) (AA 31-130) antibody
CD58 Reaktivität: Human WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-CD58 (CD58) antibody (PE)
CD58 Reaktivität: Human, Schwein FACS Wirt: Maus Monoclonal MEM-63 PE

anti-CD58 (CD58) antibody (APC)
CD58 Reaktivität: Human, Schwein FACS Wirt: Maus Monoclonal MEM-63 APC

anti-CD58 (CD58) (AA 1-241) antibody
CD58 Reaktivität: Human WB, ELISA, IHC, IHC (p), IF Wirt: Maus Monoclonal 2D11-B10 unconjugated

anti-CD58 (CD58) (AA 29-215) antibody
CD58 Reaktivität: Human FACS, WB, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

Approved: Ctox, Flo, IHC, IHC-Fr, IP

Usage: Antibody will inhibit HLA-DR mediated T cell cytotoxicity. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation. Immunohistochemistry: Frozen. Flow Cytometry: 5x10^5 cultured human Nalm-6 cells were washed and incubated 45 minutes on ice with 80 mL of anti-CD58/Biotin at 10 mg/mL. Cells were washed twice and incubated with 2? reagent Streptavidin/R-PE after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive with a mean shift of 1.29 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was 94 % blocked when pre incubated 10 minutes with 20 mL of 0.5 mg/mL anti-CD58 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare

Target Species of Antibody: Human

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

Lot specific

Buffer

PBS,  pH 7.2, 5 % glycerol, 0.2 % BSA, 0.05 % sodium azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Target

CD58

Andere Bezeichnung

CD58 (CD58 Produkte)

Hintergrund

Name/Gene ID: CD58
Family: Immunoglobulin

Synonyms: CD58, CD58 antigen, AG3, LFA-3, LFA3, Surface glycoprotein LFA-3, Surface glycoprotein lfa3, CD58 Molecule

Gen-ID

965

UniProt

P19256