GDF2 Antikörper (AA 320-429)

Produktnummer ABIN203805

Produktdetails anti-GDF2 Antikörper

Target: GDF2 (Growth Differentiation Factor 2 (GDF2))

Bindungsspezifität: AA 320-429

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GDF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut), Functional Studies (Func)

Spezifität: Recognizes human Bone Morphogenetic Protein 9. Does not cross react with recombinant human BMP-1, -1.1, -2, -3, -3b, -4, -5, -6, -7, -8 or -10. In direct ELISAs, ~80 % cross-reactivity with recombinant mouse BMP-9 and 10 % cross-reactivity with recombinant mouse BMP-10 is observed.

Aufreinigung: Affinity purified

Sterilität: 0.2 μm filtered

Immunogen: Recombinant protein corresponding to aa320-429 from human BMP-9, expressed in E.coli (UniProt# Q9UK05).

Type of Immunogen: Recombinant protein

Klon: 360107

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 1 μg/mL), Func, Neut, WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot, ELISA and Neutralization. Western Blot: 1-2 μg/mL, The detection limit is ~5 ng/lane under non-reducing conditions. Direct ELISA: 0.5-1.0 μg/mL, The detection limit is ~5 ng/well. Neutralization of human BMP-9 bioactivity: The ND50 is defined as the concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when the cytokine is present at a concentration just high enough to elicit maximum response. The ND50 of this lot is ~0.01-0.04 μg/mL in the presence of 2 ng/mL rhBMP-9 using ATDC5 chondrogenic cells.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS

Konzentration: Lot specific

Buffer: Lyophilized from a 0.2 um filtered solution in PBS, 5 % trehalose.

Handhabung: Avoid frequent freeze thaws. Store undiluted.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store at -20°C to -70°C. Stable for 1 year from date of receipt.

Details zu GDF2

Target: GDF2 (Growth Differentiation Factor 2 (GDF2))

Andere Bezeichnung: GDF2 / BMP9 (GDF2 Produkte)

Synonyme: bmp9 antikoerper, BMP-9 antikoerper, BMP9 antikoerper, Bmp9 antikoerper, DSL1 antikoerper, growth differentiation factor 2 antikoerper, gdf2 antikoerper, GDF2 antikoerper, Gdf2 antikoerper

Hintergrund: Name/Gene ID: GDF2
Family: TGF beta

Synonyms: GDF2, BMP-9, BMP9, GDF-2, Bone morphogenetic protein 9

Gen-ID: 2658

UniProt: Q9UK05

Pathways: Transition Metal Ion Homeostasis