His Tag Antikörper (iFluor 555)

Produktnummer ABIN2017573

Dieser Maus Monoklonal Antikörper detektiert spezifisch His Tag in IF.

Kurzübersicht für His Tag Antikörper (iFluor 555) (ABIN2017573)

Target: His Tag

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser His Tag Antikörper ist konjugiert mit iFluor 555

Applikation: Immunofluorescence (IF)

Klon: 6G2A9

Produktdetails anti-His Tag Antikörper (iFluor 555)

Sequenz: HHHHHH

Produktmerkmale: THETM His Tag Antibody [iFluor 555], mAb, Mouse is THETM His Tag Antibody (A00186) conjugated with iFluor 555 under optimal conditions with an F/P ratio of 3-6. It is suitable for detecting the expression level of His-tagged proteins. iFluor 555 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 555.

Aufreinigung: Protein A affinity column

Immunogen: A synthetic peptide HHHHHH coupled to KLH

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescent staining:1-4 μg/mL
Other applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid repeated freezing and thawing cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized product remains stable up to 2 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.

Haltbarkeit: 12 months

Details zu His Tag

Target: His Tag

Substanzklasse: Tag

Hintergrund: The His tag is a short amino acid sequence consists of Histidine (His) residues appended to recombinant proteins. Ni-charged resins are used for the purification of His-tagged proteins from eukaryotic and prokaryotic expression systems. An anti-His tag antibody is a useful tool for the analysis of His-tagged proteins.