Plakophilin 3 Antikörper

Produktnummer ABIN201418

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Plakophilin 3 in WB, ELISA, IHC, IP, IF und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus und Hund.

Kurzübersicht für Plakophilin 3 Antikörper (ABIN201418)

Target: Plakophilin 3 (PKP3)

Reaktivität: Human, Maus, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Plakophilin 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Plakophilin 3 Antikörper

Spezifität: Reacts with the 87kD human Plakophilin-3. There is no cross reactivity with human Plakophilin-1 and Plakophilin-2 proteins. Reactivity is confirmed with human A431 cells, and HEK293 cells transfected with plasmid expressing human Plakophilin-3. Based on sequence homology, this antibody is expected to react with mouse and canine.

Aufreinigung: Protein A purified

Immunogen: Synthetic peptide from Armadillo domain of human Plakophilin-3.

Type of Immunogen: Synthetic peptide

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.1 - 1 μg/mL), IF (1 - 3 μg/mL), IHC, IHC-P, IP, WB (1 - 3 μg/mL)

Usage: Suitable for use in ELISA, Immunoprecipitation (native), Immunohistochemistry (formalin fixed paraffin embedded), Immunofluorescence and Western Blot. ELISA: 0.1-1 μg/mL. Immunoprecipitation: 10 μg/IP reaction. Immunohistochemistry: 10 μg/mL. Immunofluorescence: 1-3 μg/mL. Western Blot: 1-3 μg/mL. Note: Immunofluorescent assays were performed using methanol-fixed HCT8/E8 and paraformaldehyde-fixed HaCat cells. For optimal IF results, methanol and paraformaldehyde- fixed cells should be permeabilized with 0.2 % Triton X-100 for 5 and 15 minutes, respectively, prior to incubation with primary antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu Plakophilin 3

Target: Plakophilin 3 (PKP3)

Andere Bezeichnung: PKP3 / Plakophilin 3

Hintergrund: Name/Gene ID: PKP3

Synonyms: PKP3, Plakophilin 3, Plakophilin-3, Plakophilin 3b

Gen-ID: 11187

UniProt: Q9Y446