Nestin Antikörper

Produktnummer ABIN201334

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Nestin in WB, IHC, ICC und IHC (fro). Er zeigt eine Reaktivität gegenüber Maus und Ratte.

Kurzübersicht für Nestin Antikörper (ABIN201334)

Target: Nestin (NES)

Reaktivität: Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nestin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Nestin Antikörper

Spezifität: Recognizes rodent Nestin at 200-220kD. Specificity confirmed with mouse and rat. Does not cross-react with human.

Aufreinigung: Purified

Immunogen: Nestin purified from embryonic rat spinal cord.

Type of Immunogen: Purified protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ICC (1:20 - 1:200), IHC, IHC-Fr (1:10 - 1:100), WB

Usage: Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Immunohistochemistry (frozen or paraffin): 1:20-1:200. Perfuse tissue with 4 % paraformaldehyde,  pH 7.4 for light microscopy or with either 4 % paraformaldehyde,  pH 10 or 4 % paraformaldehyde with 0.1 % glutaraldehyde,  pH 7.4 for EM. Immunocytochemistry: 1:20-1:200. Fix cells in 4 % paraformaldehyde buffered with 50 mM sodium borate,  pH 9.5. Western Blot: 200-220kD in reducing gels of newborn rat or mouse cell extracts. Prepare samples by boiling in 4 volumes of 125 mM Tris,  pH 6.8, 10 % 2-mercaptoethanol, 10 % glycerol, 4.6 % SDS. 5 % PAGE gels are suggested. Block membranes with milk or BSA. Do not dilute primary antibody in buffer containing milk. Freezing and thawing of samples or samples not stored at -70?C will show breakdown of nestin sometimes into multiple fragments. Positive control: Mouse embryonic brain tissue.

Not recommended for: IHC-P

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-glycine, 0.15 M sodium chloride,  pH 7.4, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Nestin

Target: Nestin (NES)

Andere Bezeichnung: Nestin

Hintergrund: Name/Gene ID: NES

Synonyms: NES, Nestin, Nbla00170

Gen-ID: 10763

UniProt: P48681