ITGA6 Antikörper

Produktnummer ABIN201115

Dieses Maus Monoklonal-Antikörper erkennt spezifisch ITGA6 in WB, IHC, IP und ICC. Er zeigt eine Reaktivität gegenüber Huhn.

Kurzübersicht für ITGA6 Antikörper (ABIN201115)

Target: ITGA6 (Integrin, alpha 6 (ITGA6))

Reaktivität: Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ITGA6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-ITGA6 Antikörper

Spezifität: Reactive with chicken integrin alpha6, as demonstrated by experiments with cDNA-transfected NIH3T3 cells followed by immunoblots.

Aufreinigung: Purified

Immunogen: Integrin alpha subunits purified from adult chicken brain tissue, denatured in 6 M guanidine isothiocyanate, and depleted of beta1 subunit.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IHC, IP, WB

Usage: Western Blot. Immunoprecipitation: Precipitates the monomeric alpha6 subunit after the dimeric receptor has been denatured, but does not precipitate the native receptor. Immunohistochemistry: Methanol-fixed cryosections. An overnight methanol fix is suggested. Previous experiments have used 14 micron sections of chicken embryonic tissue. Typical antibodies dilutions are 35 μg/mL, with overnight antibody incubation, followed by detection with a goat anti-mouse IgG-FITC secondary antibody. Immunocytochemistry: Methanol-fixed cultured cells (15 minute fixation). Western Blot: Recognizes a 130kD band in chicken brain tissue and a 140kD band in transfected NIH3T3 cells (the difference in molecular weight is likely due to differential glycosylation).

Kommentare:

Target Species of Antibody: Chicken

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.6, 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu ITGA6

Target: ITGA6 (Integrin, alpha 6 (ITGA6))

Andere Bezeichnung: ITGA6/Integrin Alpha 6/CD49f

Hintergrund: Name/Gene ID: ITGA6
Family: Integrin

Synonyms: ITGA6, CD49f, CD49f antigen, Integrin alpha6B, Integrin, alpha 6, Integrin alpha-6 precursor, VLA-6, Integrin alpha-6, ITGA6B

Gen-ID: 3655

UniProt: P23229

Pathways: CXCR4-mediated Signaling Events, Brown Fat Cell Differentiation, Integrin Complex