BUB1B Antikörper (AA 1-350)

Produktnummer ABIN199935

Dieses Anti-BUB1B-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von BUB1B in WB, IF und IP. Geeignet für Human.

Kurzübersicht für BUB1B Antikörper (AA 1-350) (ABIN199935)

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BUB1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Produktdetails anti-BUB1B Antikörper

Bindungsspezifität: AA 1-350

Spezifität: Amino acid residues 1-350 of human BUBR1 Kinase protein.

Aufreinigung: Protein A purified

Immunogen: Recombinant human BUB1B / BubR1.

Type of Immunogen: Recombinant protein

Anwendungsinformationen

Applikationshinweise: Approved: IF, IP, WB

Usage: This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~120 kD corresponding to full-length protein by western blotting in the appropriate cell lysate or extract. Higher MW bands may be seen that may be due to hyper-phosphorylation of the protein. The use of HeLa whole cell lysates is recommended as a positive control. For IF microscopy use cells grown on cover slips fixed with 3.5 % paraformaldehyde in PBS at  pH 6.8. Permeabilize fixed cells with 0.2 % Triton X-100 in 25 mM Tris Cl,  pH 7.4 containing 0.1 % BSA.

Not recommended for: IHC-P

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu BUB1B

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Andere Bezeichnung: BUB1B / BubR1

Hintergrund: Name/Gene ID: BUB1B
Subfamily: BUB1
Family: Protein Kinase

Synonyms: BUB1B, Bub1A, BUBR1, HBUBR1, Protein SSK1, SSK1, BUB1beta, MAD3L

Gen-ID: 701

UniProt: O60566