TYR Antikörper (AA 1-433)

Produktnummer ABIN199780

Dieses Maus Monoklonal-Antikörper erkennt spezifisch TYR in WB, IHC, IF, IHC (p) und ICC. Er zeigt eine Reaktivität gegenüber Human, Hund und Cat.

Kurzübersicht für TYR Antikörper (AA 1-433) (ABIN199780)

Target: TYR (Tyrosinase (TYR))

Reaktivität: Human, Hund, Cat

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TYR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Produktdetails anti-TYR Antikörper

Bindungsspezifität: AA 1-433

Spezifität: Recognizes a cluster of proteins between 70-80kD, identified as tyrosinase. Occasionally a minor band at 55kD is also detected. Does not cross-react with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Species cross-reactivity: Human, canine and feline. Weak cross-reactivity with mouse.

Aufreinigung: Protein A purified

Immunogen: Recombinant tyrosinase protein. Epitope: 1-433. Cellular Localization: Cytoplasmic.

Type of Immunogen: Recombinant protein

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IF, IHC, IHC-P, WB (1 - 2 μg/mL)

Usage: Suitable for use in Immunocytology, Immunofluorescence, Western Blot and Immunohistology. Western Blot: 1-2 μg/mL for 2 hours at RT. Immunohistology: 2-4 μg/mL for 30 minutes at RT (formalin/paraffin). Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA,  pH 8.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: Melanoma cell lines (SK-MEL-13, SK-MEL-19, SK-MEL-30, SK-MEL-37), melanocytes in skin and Melanomas (1). The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4. No preservatives or stabilizing proteins added.

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu TYR

Target: TYR (Tyrosinase (TYR))

Andere Bezeichnung: Tyrosinase

Hintergrund: Name/Gene ID: TYR

Synonyms: TYR, CMM8, LB24-AB, SHEP3, SK29-AB, Monophenol monooxygenase, Tumor rejection antigen AB, OCA1A, Tyrosinase, OCAIA

Gen-ID: 7299

UniProt: P14679