CD51/CD61 Antikörper

Produktnummer ABIN199326

Dieses Anti-CD51/CD61-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD51/CD61 in FACS, IP, IHC (fro), IF, IHC und ICC. Geeignet für Human, Huhn, Kaninchen, Rind (Kuh), Hund, Affe, Schwein und Avian.

Kurzübersicht für CD51/CD61 Antikörper (ABIN199326)

Target: CD51/CD61 (ITGAV/ITGB3) (Integrin alpha V, Integrin beta 3 (ITGAV/ITGB3))

Reaktivität: Human, Huhn, Kaninchen, Rind (Kuh), Hund, Affe, Schwein, Avian

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD51/CD61 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Klon: LM609

Produktdetails anti-CD51/CD61 Antikörper

Spezifität: Recognizes the human vitronectin receptor aVb3 complex, an RGD-directed adhesion receptor. Species cross-reactivity: bovine, chicken, monkey, porcine, canine, rabbit and avian. Not reactive with rat tissue.

Aufreinigung: Protein A purified

Immunogen: Purified adhesion receptor from the human melanoma cell line M21.

Type of Immunogen: Cells

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: Flo, ICC, IF (5 - 10 μg/mL), IHC, IHC-Fr, IP

Usage: Suitable for use in Immunoprecipitation, Immunofluorescence, Flow Cytometry, Immunohistochemistry (frozen sections only), Immunocytochemistry and Blocking. Immunoprecipitation: Precipitates integrin alpha V beta3 complex. When resolved by SDS-PAGE under reducing conditions, bands are observed at ~130kD corresponding to the alpha V subunit and at ~105kD corresponding to the beta3 subunit. Flow Cytometry: 1-3 μg/0.5x1E6cells in 100 μL volume. Immunofluorescence: 5-10 μg/mL for staining of bFGF-treated 6mm cryosections of chick chorioallantoic membrane fixed with acetone. Also effective on 4 % paraformaldehyde fixed, frozen tissues and cells. Not effective for immunohistochemical staining of paraffin-embedded tissue sections. Blocking: Inhibits adhesion of cells to vitronectin coated surfaces at 10-25 μg/mL. Positive control: Human myeloma cell lines M21 and MoalphaV.

Not recommended for: IHC-P

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.1. No preservatives added.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu CD51/CD61

Target: CD51/CD61 (ITGAV/ITGB3) (Integrin alpha V, Integrin beta 3 (ITGAV/ITGB3))

Andere Bezeichnung: Integrin Alpha V Beta 3