EGLN3 Antikörper (AA 2-239) (FITC)

Produktnummer ABIN1992142

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch EGLN3 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für EGLN3 Antikörper (AA 2-239) (FITC) (ABIN1992142)

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EGLN3 Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-EGLN3 Antikörper (FITC)

Bindungsspezifität: AA 2-239

Kreuzreaktivität: Maus

Aufreinigung: >95%, Protein G purified

Immunogen: Recombinant Mouse Egl nine homolog 3 protein (2-239AA)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working concentration should be determinde by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin^TM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freeze.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Details zu EGLN3

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Andere Bezeichnung: Egln3

Hintergrund:

Background: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. ELGN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex. In neurons, has a NGF-induced proapoptotic effect, probably through regulating CASP3 activity. Also essential for hypoxic regulation of neutrophilic inflammation.

Aliases: Egln3Egl nine homolog 3 antibody, EC 1.14.11.29 antibody, Hypoxia-inducible factor prolyl hydroxylase 3 antibody, HIF-PH3 antibody, HIF-prolyl hydroxylase 3 antibody, HPH-3 antibody, Prolyl hydroxylase domain-containing protein 3 antibody, PHD3 antibody, SM-20 antibody

UniProt: Q91UZ4

Pathways: Positive Regulation of Endopeptidase Activity