Selectin E/CD62e Antikörper

Produktnummer ABIN198855

Produktdetails anti-Selectin E/CD62e Antikörper

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reaktivität: Human, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Selectin E/CD62e Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Reacts with E-selectin CD62-E, previous designated the Endothelial Leucocyte Adhesion Molecule-1 (ELAM-1). Reacts with human endothelial cells activated with TNF-a, IL-1 or endotoxin. Found to react also with cells transfected with the E-selectin gene. Inhibits the adhesion of granulocytes both neutrophilic and eosinophilic.

Aufreinigung: Purified

Immunogen:

Type of Immunogen: Native protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-Fr, IP

Usage: The antibody is useful for staining of E-selectin expressing endothelial cells. It permits staining of in vitro cultured cells as well as frozen sections. The antibody can also be used for staining of chimpanzee, rhesus monkey, cynomolgus monkey and baboon endothelial cells expressing E-selectin. Furthermore the antibody can be used for Immunoprecipitation and immunoassays. Immunohistology: 1:10. In vitro cultured cells can be fixed with 1 % paraformaldehyde and kept in PBS plus azide before staining. Tissue sections are advised to be fixed for 10 min in pure acetone and followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised to be followed by a biotin conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNF-a (1 ng/mL), IL-1 (100U/mL) or LPS (1 μg/mL) in tissue culture medium at 37 C. As negative control it is advised to use a control murine IgG1 antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.02 % sodium azide, 0.1 % BSA.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu Selectin E/CD62e

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Andere Bezeichnung: SELE / CD62E / E-selectin (SELE Produkte)

Synonyme: SELE antikoerper, CD62E antikoerper, ELAM antikoerper, ELAM1 antikoerper, ESEL antikoerper, LECAM2 antikoerper, E-selectin antikoerper, Elam antikoerper, selectin E antikoerper, selectin, endothelial cell antikoerper, SELE antikoerper, Sele antikoerper

Hintergrund: Name/Gene ID: SELE

Synonyms: SELE, CD62E, ELAM, ESEL, E-selectin, LECAM2, CD62E antigen, ELAM-1, ELAM1, Selectin E

Gen-ID: 6401

UniProt: P16581

Pathways: Thromboxane A2 Receptor Signaling