CD1c Antikörper (PE)

Produktnummer ABIN1981893

Produktdetails anti-CD1c Antikörper (PE)

Target: CD1c (CD1C) (T-cell surface glycoprotein CD1c (CD1C))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD1c Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Spezifität: The mouse monoclonal antibody L161 recognizes an extracellular epitope of CD1c, (R7), a 43 kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells, B and some T cells.

Kreuzreaktivität (Details): Human

Aufreinigung: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: human thymocytes

Klon: L161

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Kommentare:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze. Avoid prolonged exposure to light.
Do not use after expiration date stamped on vial label.
Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-CD1c Antikörper (PE) (ABIN1981893)

Scharf, Li, Hawk, Garzón, Zhang, Fox, Kazen, Shah, Haddadian, Gumperz, Saghatelian, Faraldo-Gómez, Meredith, Piccirilli, Adams: "The 2.5 Å structure of CD1c in complex with a mycobacterial lipid reveals an open groove ideally suited for diverse antigen presentation." in: Immunity, Vol. 33, Issue 6, pp. 853-62, (2010) (PubMed).

del C Salamone, Mendiguren, Salamone, Fainboim: "Membrane trafficking of CD1c on activated T cells." in: Journal of leukocyte biology, Vol. 70, Issue 4, pp. 567-77, (2001) (PubMed).

Briken, Jackman, Watts, Rogers, Porcelli: "Human CD1b and CD1c isoforms survey different intracellular compartments for the presentation of microbial lipid antigens." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 281-8, (2000) (PubMed).

Todate, Chida, Suda, Imokawa, Sato, Ide, Tsuchiya, Inui, Nakamura, Asada, Hayakawa, Nakamura: "Increased numbers of dendritic cells in the bronchiolar tissues of diffuse panbronchiolitis." in: American journal of respiratory and critical care medicine, Vol. 162, Issue 1, pp. 148-53, (2000) (PubMed).

Details zu CD1c

Target: CD1c (CD1C) (T-cell surface glycoprotein CD1c (CD1C))

Andere Bezeichnung: CD1c (CD1C Produkte)

Hintergrund: CD1c molecule,CD1c (also known as R7 or BDCA1) together with CD1a and b, belongs to group 1 of CD1 antigens. These non-classical MHC-like glycoproteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. The trafficking routes of the particular CD1 types differ and correspond to their ability to bind and present different groups of antigens. CD1c is unique in its ability to present e.g. mycobacterial phosphoketides and polyisoprenoids. CD1c is the only CD1 isoform that has been shown to interact both with alpha/beta and gamma/delta T cells.,R7, BDCA1

Gen-ID: 911

UniProt: P29017