MARCKS antibody detects endogenous levels of total MARCKS protein.
Aufreinigung
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human MARCKS around the phosphorylation site of serine 162 (K-K-SP-F-K).
MARCKS
Reaktivität: Human
WB
Wirt: Kaninchen
Polyclonal
RB04646
unconjugated
Applikationshinweise
Western Blot: 1: 500approx. 1: 1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
-20 °C
Target
MARCKS
(Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))
Andere Bezeichnung
MARCKS
Hintergrund
MARCKS, (Myristoylated Alanine-Rich C Kinase Substrate), is a member of a family of calmodulin binding proteins and is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin.Synonyms: MACS, Myristoylated alanine-rich C-kinase substrate, PRKCSL