IRS1 Antikörper (Ser636)

Produktnummer ABIN197466

Dieses Anti-IRS1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von IRS1 in WB, IF und IHC (p). Geeignet für Human, Maus und Ratte.

Kurzübersicht für IRS1 Antikörper (Ser636) (ABIN197466)

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IRS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-IRS1 Antikörper

Bindungsspezifität: Ser636

Spezifität: This antibody AP02732PU detects endogenous levels of total IRS-1 protein.

Aufreinigung: Immunoaffinity Chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 636 (P-M-SP-P-K).

Anwendungsinformationen

Applikationshinweise: Western blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu IRS1

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Andere Bezeichnung: IRS1

Hintergrund: Insulin receptor substrates (IRS) are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. Serine/threonine phosphorylation of IRS1 has been demonstrated to be a negative regulator of insulin signaling and is responsible for its degradation, although IRS1 degradation pathways are not well understood. IRS1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm's tumors, and adrenal cortical carcinomas, thus making IRS1 phosphorylation and subsequent degradation an attractive therapeutic target. To date there have been four subtypes identified: IRS1, 2, 3 and 4, with IRS1 being widely expressed.Synonyms: IRS-1, Insulin receptor substrate 1

Gen-ID: 3667

NCBI Accession: NP_005535

UniProt: P35568

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process