HSF1 Antikörper (Ser307)

Produktnummer ABIN197434

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch HSF1 in WB und IHC (p). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für HSF1 Antikörper (Ser307) (ABIN197434)

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-HSF1 Antikörper

Bindungsspezifität: Ser307

Spezifität: HSF1 antibody detects endogenous levels of total HSF1 protein.

Aufreinigung: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human HSF1 around the phosphorylation site of serine 307 (P-Q-SP-P-R).

Anwendungsinformationen

Applikationshinweise: Western Blot: 1: 500approx. 1: 1000. Immunohistochemistry on paraffin sections: 1: 50-1: 100.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Andere Bezeichnung: Heat Shock Factor 1 / HSF1

Hintergrund: All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF 1 and HSF 2, which bind to the same HSEs and have 38 % sequence identity. These factors are activated by distinct stimuli, HSF 1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF 2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF 1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF 1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF 1 is necessary for maximal transcription of heat shock genes.Synonyms: HSF-1, HSTF1, Heat shock transcription factor 1

Gen-ID: 3297

NCBI Accession: NP_005517

UniProt: Q00613