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APP Antikörper (AA 666-670)

This anti-APP antibody (ABIN197432) is a Rabbit Polyclonal antibody detecting APP in WB, IF. Suitable for Human, Mouse, Rat.
Produktnummer ABIN197432

Quick Overview for APP Antikörper (AA 666-670) (ABIN197432)

Target

Alle APP Antikörper anzeigen
APP (Amyloid beta (A4) Precursor Protein (APP))

Reaktivität

  • 259
  • 116
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  • 1
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Human, Maus, Ratte

Wirt

  • 214
  • 86
  • 4
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  • 3
  • 2
Kaninchen

Klonalität

  • 231
  • 80
  • 1
Polyklonal

Konjugat

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  • 7
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  • 2
  • 2
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  • 2
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  • 2
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Dieser APP Antikörper ist unkonjugiert

Applikation

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  • 2
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  • 1
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  • 1
Western Blotting (WB), Immunofluorescence (IF)
  • Bindungsspezifität

    • 30
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    • 1
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    • 1
    • 1
    • 1
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    • 1
    • 1
    • 1
    AA 666-670

    Spezifität

    This antibody detects endogenous levels of total APP protein.

    Aufreinigung

    Affinity Chromatography using epitope-specific immunogen.

    Immunogen

    peptide Sequence around amino acids 666~670 (A-V-T-P-E) derived from Human APP protein.
  • Applikationshinweise

    Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Validierung #029576 (Immunofluorescence)
    'Independent Validation' Siegel
    by
    Molecular Pathology Core
    No.
    #029576
    Datum
    11.01.2014
    Antigen
    Chargennummer
    8715
    Validierte Anwendung
    Immunofluorescence
    Positivkontrolle
    Human brain
    Negativkontrolle
    Human liver
    Bewertung
    Signal was detected in positive control tissue, and no signal was seen in negative control tissue.
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    • Antibody: Amyloid beta A4 precursor protein (APP)
    • Catalog number: ABIN197433
    • Lot number: 8715
    Sekundärantikörper
    • Antibody: AlexaFluor 488 goat anti-Rabbit IgG
    • Lot number: 702323
    Full Protocol
    • Sections were deparaffinized and rehydrated.
    • Sections were heated to 98°C for 20 min in citrate buffer pH 6.0 (Biogenex, HK086-9K) for antigen retrieval and cooled down for 20 min on the bench.
    • Sections were blocked in 10% NGS (normal goat serum) for 20 min at room temperature.
    • Sections were washed x 2 in 1xTBS buffer.
    • Sections were incubated with primary antibody diluted 1:100 in Antibody Diluent (Invitrogen, 003218) at 4°C overnight.
    • Sections were washed x 2 in 1xTBS buffer.
    • Sections were incubated with AlexaFluor 488 Goat anti-Rabbit IgG 1:1000 for 60 min.
    • Sections were washed x 3 in 1xTBS buffer.
    • Sections were mounted with DAPI (Invitrogen, Prolong Gold antifade reagent with DAPI) and coverslipped.
    • Sections were photographed with a Zeiss Axioskop2 microscope
    Anmerkungen
    None
  • Konzentration

    1.0 mg/mL

    Buffer

    PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Store the antibody (in aliquots) at -20 °C.
  • Target

    APP (Amyloid beta (A4) Precursor Protein (APP))

    Andere Bezeichnung

    Amyloid beta A4 Protein / APP

    Hintergrund

    Amyloid beta precursor protein gene (ABPP) encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Multiple transcript variants encoding several different isoforms have been found for this gene. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in nonneuronal cells. Isoform APP751 is the most abundant form in T lymphocytes. ABPP is expressed in all fetal tissues examined with the highest levels in brain, kidney, heart and spleen with weak expression observed in liver, ABPP is induced during neuronal differentiation. In the adult brain, highest expression of ABPP gene is found in the frontal lobe of the cortex and in the anterior perisylvian cortex opercular gyri, moderate expression in the cerebellar cortex, the posterior perisylvian cortex opercular gyri and the temporal associated cortex. Weak expression is found in the striate, extra striate and motor cortices. Mutations in ABPP have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy).Synonyms: ABPP, APPI, Alzheimer disease amyloid protein, Amyloid Precursor Protein, CVAP, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II

    Gen-ID

    351

    NCBI Accession

    NP_000475

    UniProt

    P05067

    Pathways

    Caspase Kaskade in der Apoptose, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour
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