APP Antikörper (AA 666-670)

Produktnummer ABIN197432

Dieses Anti-APP-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von APP in WB und IF. Geeignet für Human, Maus und Ratte. Unabhängig validiert für die Verwendung in Immunofluorescence.

Kurzübersicht für APP Antikörper (AA 666-670) (ABIN197432)

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser APP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktdetails anti-APP Antikörper

Bindungsspezifität: AA 666-670

Spezifität: This antibody detects endogenous levels of total APP protein.

Aufreinigung: Affinity Chromatography using epitope-specific immunogen.

Immunogen: peptide Sequence around amino acids 666~670 (A-V-T-P-E) derived from Human APP protein.

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (IF)

Validierung #029576 (Immunofluorescence)

by: Molecular Pathology Core

No.: #029576

Datum: 11.01.2014

Chargennummer: 8715

Validierte Anwendung: Immunofluorescence

Positivkontrolle: Human brain

Negativkontrolle: Human liver

Bewertung: Signal was detected in positive control tissue, and no signal was seen in negative control tissue.

Validierungsbilder

Figure 1. Micrograph image of positive control (human brain FFPE tissue). APP staining appears in green.

Figure 2: micrograph image of negative control (human liver FFPE tissue) stained with APP antibody.

Figure 3: micrograph image of isotype control (rabbit IgG isotype control antibody on human brain FFPE tissue).

Figure 4: micrograph image of secondary antibody only control (no primary antibody on human brain FFPE tissue).

Protokoll

Primärantikörper:

• Antibody: Amyloid beta A4 precursor protein (APP)
• Catalog number: ABIN197433
• Lot number: 8715

Sekundärantikörper:

• Antibody: AlexaFluor 488 goat anti-Rabbit IgG
• Lot number: 702323

Full Protocol:

• Sections were deparaffinized and rehydrated.
• Sections were heated to 98°C for 20 min in citrate buffer pH 6.0 (Biogenex, HK086-9K) for antigen retrieval and cooled down for 20 min on the bench.
• Sections were blocked in 10% NGS (normal goat serum) for 20 min at room temperature.
• Sections were washed x 2 in 1xTBS buffer.
• Sections were incubated with primary antibody diluted 1:100 in Antibody Diluent (Invitrogen, 003218) at 4°C overnight.
• Sections were washed x 2 in 1xTBS buffer.
• Sections were incubated with AlexaFluor 488 Goat anti-Rabbit IgG 1:1000 for 60 min.
• Sections were washed x 3 in 1xTBS buffer.
• Sections were mounted with DAPI (Invitrogen, Prolong Gold antifade reagent with DAPI) and coverslipped.
• Sections were photographed with a Zeiss Axioskop2 microscope

Anmerkungen: None

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu APP

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Andere Bezeichnung: Amyloid beta A4 Protein / APP

Hintergrund: Amyloid beta precursor protein gene (ABPP) encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Multiple transcript variants encoding several different isoforms have been found for this gene. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in nonneuronal cells. Isoform APP751 is the most abundant form in T lymphocytes. ABPP is expressed in all fetal tissues examined with the highest levels in brain, kidney, heart and spleen with weak expression observed in liver, ABPP is induced during neuronal differentiation. In the adult brain, highest expression of ABPP gene is found in the frontal lobe of the cortex and in the anterior perisylvian cortex opercular gyri, moderate expression in the cerebellar cortex, the posterior perisylvian cortex opercular gyri and the temporal associated cortex. Weak expression is found in the striate, extra striate and motor cortices. Mutations in ABPP have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy).Synonyms: ABPP, APPI, Alzheimer disease amyloid protein, Amyloid Precursor Protein, CVAP, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II

Gen-ID: 351

NCBI Accession: NP_000475

UniProt: P05067

Pathways: Caspase Kaskade in der Apoptose, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour